CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease

CD25+CD4+ regulatory T cells participate in the regulation of immune responses. We recently demonstrated the presence of CD25brightCD4+ regulatory T cells with a capacity to control T cell proliferation in the joints of patients with rheumatoid arthritis. Here, we investigate a possible accumulation...

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Main Authors:	Cao, Duojia, Vollenhoven, Ronald van, Klareskog, Lars, Trollmo, Christina, Malmström, Vivianne
Format:	Online
Language:	English
Published:	BioMed Central 2004
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC464877/

id	pubmed-464877
recordtype	oai_dc
spelling	pubmed-4648772004-07-16 CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease Cao, Duojia Vollenhoven, Ronald van Klareskog, Lars Trollmo, Christina Malmström, Vivianne Research Article CD25+CD4+ regulatory T cells participate in the regulation of immune responses. We recently demonstrated the presence of CD25brightCD4+ regulatory T cells with a capacity to control T cell proliferation in the joints of patients with rheumatoid arthritis. Here, we investigate a possible accumulation of these regulatory T cells in the inflamed joint of different rheumatic diseases including rheumatoid arthritis. The studies are also extended to analyze whether cytokine production can be suppressed by the regulatory T cells. Synovial fluid and peripheral blood samples were obtained during relapse from 36 patients with spondyloarthropathies, 21 adults with juvenile idiopathic arthritis and 135 patients with rheumatoid arthritis, and the frequency of CD25brightCD4+ T cells was determined. Of 192 patients, 182 demonstrated a higher frequency of CD25brightCD4+ T cells in synovial fluid than in peripheral blood. In comparison with healthy subjects, the patients had significantly fewer CD25brightCD4+ T cells in peripheral blood. For functional studies, synovial fluid cells from eight patients were sorted by flow cytometry, and the suppressive capacity of the CD25brightCD4+ T cells was determined in in vitro cocultures. The CD25brightCD4+ T cells suppressed the production of both type 1 and 2 cytokines including interleukin-17, as well as proliferation, independently of diagnosis. Thus, irrespective of the inflammatory joint disease investigated, CD25brightCD4+ T cells were reduced in peripheral blood and enriched in the joint, suggesting an active recruitment of regulatory T cells to the affected joint. Their capacity to suppress both proliferation and cytokine secretion might contribute to a dampening of local inflammatory processes. BioMed Central 2004 2004-06-07 /pmc/articles/PMC464877/ /pubmed/15225369 http://dx.doi.org/10.1186/ar1192 Text en Copyright © 2004 Cao et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Cao, Duojia Vollenhoven, Ronald van Klareskog, Lars Trollmo, Christina Malmström, Vivianne
spellingShingle	Cao, Duojia Vollenhoven, Ronald van Klareskog, Lars Trollmo, Christina Malmström, Vivianne CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease
author_facet	Cao, Duojia Vollenhoven, Ronald van Klareskog, Lars Trollmo, Christina Malmström, Vivianne
author_sort	Cao, Duojia
title	CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease
title_short	CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease
title_full	CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease
title_fullStr	CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease
title_full_unstemmed	CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease
title_sort	cd25brightcd4+ regulatory t cells are enriched in inflamed joints of patients with chronic rheumatic disease
description	CD25+CD4+ regulatory T cells participate in the regulation of immune responses. We recently demonstrated the presence of CD25brightCD4+ regulatory T cells with a capacity to control T cell proliferation in the joints of patients with rheumatoid arthritis. Here, we investigate a possible accumulation of these regulatory T cells in the inflamed joint of different rheumatic diseases including rheumatoid arthritis. The studies are also extended to analyze whether cytokine production can be suppressed by the regulatory T cells. Synovial fluid and peripheral blood samples were obtained during relapse from 36 patients with spondyloarthropathies, 21 adults with juvenile idiopathic arthritis and 135 patients with rheumatoid arthritis, and the frequency of CD25brightCD4+ T cells was determined. Of 192 patients, 182 demonstrated a higher frequency of CD25brightCD4+ T cells in synovial fluid than in peripheral blood. In comparison with healthy subjects, the patients had significantly fewer CD25brightCD4+ T cells in peripheral blood. For functional studies, synovial fluid cells from eight patients were sorted by flow cytometry, and the suppressive capacity of the CD25brightCD4+ T cells was determined in in vitro cocultures. The CD25brightCD4+ T cells suppressed the production of both type 1 and 2 cytokines including interleukin-17, as well as proliferation, independently of diagnosis. Thus, irrespective of the inflammatory joint disease investigated, CD25brightCD4+ T cells were reduced in peripheral blood and enriched in the joint, suggesting an active recruitment of regulatory T cells to the affected joint. Their capacity to suppress both proliferation and cytokine secretion might contribute to a dampening of local inflammatory processes.
publisher	BioMed Central
publishDate	2004
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC464877/
_version_	1611369020171223040

CD25brightCD4+ regulatory T cells are enriched in inflamed joints of patients with chronic rheumatic disease

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