Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis
Necrotizing enterocolitis (NEC) is the most common gastrointestinal (GI) medical/surgical emergency of the newborn and a leading cause of preterm neonate morbidity and mortality. NEC is a challenge to diagnose since it often shares similar clinical features with neonatal sepsis. In the present study...
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Nature Publishing Group
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642514/ |
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pubmed-46425142015-11-20 Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis Tao, Guo-Zhong Liu, Bo Zhang, Rong Liu, Gigi Abdullah, Fizan Harris, Mary Cay Brandt, Mary L. Ehrenkranz, Richard A. Bowers, Corinna Martin, Camilia R. Moss, R. Lawrence Sylvester, Karl G. Article Necrotizing enterocolitis (NEC) is the most common gastrointestinal (GI) medical/surgical emergency of the newborn and a leading cause of preterm neonate morbidity and mortality. NEC is a challenge to diagnose since it often shares similar clinical features with neonatal sepsis. In the present study, plasma protein profiling was compared among NEC, sepsis and control cohorts using gel electrophoresis, immunoblot and mass spectrometry. We observed significant impairment in the formation of fibrinogen-γ dimers (FGG-dimer) in the plasma of newborns with NEC that could efficiently differentiate NEC and sepsis with a high level of sensitivity and specificity. Interestingly, the impaired FGG-dimer formation could be restored in NEC plasma by the addition of exogenous active factor XIII (FXIII). Enzymatic activity of FXIII was determined to be significantly lower in NEC subject plasma for crosslinking FGG when compared to sepsis. These findings demonstrate a potential novel biomarker and related biologic mechanism for diagnosing NEC, as well as suggest a possible therapeutic strategy. Nature Publishing Group 2015-08-17 /pmc/articles/PMC4642514/ /pubmed/26277871 http://dx.doi.org/10.1038/srep13119 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Tao, Guo-Zhong Liu, Bo Zhang, Rong Liu, Gigi Abdullah, Fizan Harris, Mary Cay Brandt, Mary L. Ehrenkranz, Richard A. Bowers, Corinna Martin, Camilia R. Moss, R. Lawrence Sylvester, Karl G. |
| spellingShingle |
Tao, Guo-Zhong Liu, Bo Zhang, Rong Liu, Gigi Abdullah, Fizan Harris, Mary Cay Brandt, Mary L. Ehrenkranz, Richard A. Bowers, Corinna Martin, Camilia R. Moss, R. Lawrence Sylvester, Karl G. Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis |
| author_facet |
Tao, Guo-Zhong Liu, Bo Zhang, Rong Liu, Gigi Abdullah, Fizan Harris, Mary Cay Brandt, Mary L. Ehrenkranz, Richard A. Bowers, Corinna Martin, Camilia R. Moss, R. Lawrence Sylvester, Karl G. |
| author_sort |
Tao, Guo-Zhong |
| title |
Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis |
| title_short |
Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis |
| title_full |
Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis |
| title_fullStr |
Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis |
| title_full_unstemmed |
Impaired Activity of Blood Coagulant Factor XIII in Patients with Necrotizing Enterocolitis |
| title_sort |
impaired activity of blood coagulant factor xiii in patients with necrotizing enterocolitis |
| description |
Necrotizing enterocolitis (NEC) is the most common gastrointestinal (GI) medical/surgical emergency of the newborn and a leading cause of preterm neonate morbidity and mortality. NEC is a challenge to diagnose since it often shares similar clinical features with neonatal sepsis. In the present study, plasma protein profiling was compared among NEC, sepsis and control cohorts using gel electrophoresis, immunoblot and mass spectrometry. We observed significant impairment in the formation of fibrinogen-γ dimers (FGG-dimer) in the plasma of newborns with NEC that could efficiently differentiate NEC and sepsis with a high level of sensitivity and specificity. Interestingly, the impaired FGG-dimer formation could be restored in NEC plasma by the addition of exogenous active factor XIII (FXIII). Enzymatic activity of FXIII was determined to be significantly lower in NEC subject plasma for crosslinking FGG when compared to sepsis. These findings demonstrate a potential novel biomarker and related biologic mechanism for diagnosing NEC, as well as suggest a possible therapeutic strategy. |
| publisher |
Nature Publishing Group |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642514/ |
| _version_ |
1613500339295617024 |