Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis
•Placental perfusion yields large amounts of STBEV.•Differential centrifugation isolates enriched preparations of STBMV and STBEX.•fl-NTA can be optimised using an EV ‘standard’.•fl-NTA reliably detects STBMV using the specific marker PLAP.
| Main Authors: | , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Academic Press
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641874/ |