Imaging fictive locomotor patterns in larval Drosophila

We have established a preparation in larval Drosophila to monitor fictive locomotion simultaneously across abdominal and thoracic segments of the isolated CNS with genetically encoded Ca2+ indicators. The Ca2+ signals closely followed spiking activity measured electrophysiologically in nerve roots....

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Main Authors:	Pulver, Stefan R., Bayley, Timothy G., Taylor, Adam L., Berni, Jimena, Bate, Michael, Hedwig, Berthold
Format:	Online
Language:	English
Published:	American Physiological Society 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637366/

id	pubmed-4637366
recordtype	oai_dc
spelling	pubmed-46373662015-11-30 Imaging fictive locomotor patterns in larval Drosophila Pulver, Stefan R. Bayley, Timothy G. Taylor, Adam L. Berni, Jimena Bate, Michael Hedwig, Berthold Call for Papers We have established a preparation in larval Drosophila to monitor fictive locomotion simultaneously across abdominal and thoracic segments of the isolated CNS with genetically encoded Ca2+ indicators. The Ca2+ signals closely followed spiking activity measured electrophysiologically in nerve roots. Three motor patterns are analyzed. Two comprise waves of Ca2+ signals that progress along the longitudinal body axis in a posterior-to-anterior or anterior-to-posterior direction. These waves had statistically indistinguishable intersegmental phase delays compared with segmental contractions during forward and backward crawling behavior, despite being ∼10 times slower. During these waves, motor neurons of the dorsal longitudinal and transverse muscles were active in the same order as the muscle groups are recruited during crawling behavior. A third fictive motor pattern exhibits a left-right asymmetry across segments and bears similarities with turning behavior in intact larvae, occurring equally frequently and involving asymmetry in the same segments. Ablation of the segments in which forward and backward waves of Ca2+ signals were normally initiated did not eliminate production of Ca2+ waves. When the brain and subesophageal ganglion (SOG) were removed, the remaining ganglia retained the ability to produce both forward and backward waves of motor activity, although the speed and frequency of waves changed. Bilateral asymmetry of activity was reduced when the brain was removed and abolished when the SOG was removed. This work paves the way to studying the neural and genetic underpinnings of segmentally coordinated motor pattern generation in Drosophila with imaging techniques. American Physiological Society 2015-08-26 2015-11 /pmc/articles/PMC4637366/ /pubmed/26311188 http://dx.doi.org/10.1152/jn.00731.2015 Text en Copyright © 2015 the American Physiological Society http://creativecommons.org/licenses/by/3.0/deed.en_US Licensed under Creative Commons Attribution CC-BY 3.0 (http://creativecommons.org/licenses/by/3.0/deed.en_US) : © the American Physiological Society.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Pulver, Stefan R. Bayley, Timothy G. Taylor, Adam L. Berni, Jimena Bate, Michael Hedwig, Berthold
spellingShingle	Pulver, Stefan R. Bayley, Timothy G. Taylor, Adam L. Berni, Jimena Bate, Michael Hedwig, Berthold Imaging fictive locomotor patterns in larval Drosophila
author_facet	Pulver, Stefan R. Bayley, Timothy G. Taylor, Adam L. Berni, Jimena Bate, Michael Hedwig, Berthold
author_sort	Pulver, Stefan R.
title	Imaging fictive locomotor patterns in larval Drosophila
title_short	Imaging fictive locomotor patterns in larval Drosophila
title_full	Imaging fictive locomotor patterns in larval Drosophila
title_fullStr	Imaging fictive locomotor patterns in larval Drosophila
title_full_unstemmed	Imaging fictive locomotor patterns in larval Drosophila
title_sort	imaging fictive locomotor patterns in larval drosophila
description	We have established a preparation in larval Drosophila to monitor fictive locomotion simultaneously across abdominal and thoracic segments of the isolated CNS with genetically encoded Ca2+ indicators. The Ca2+ signals closely followed spiking activity measured electrophysiologically in nerve roots. Three motor patterns are analyzed. Two comprise waves of Ca2+ signals that progress along the longitudinal body axis in a posterior-to-anterior or anterior-to-posterior direction. These waves had statistically indistinguishable intersegmental phase delays compared with segmental contractions during forward and backward crawling behavior, despite being ∼10 times slower. During these waves, motor neurons of the dorsal longitudinal and transverse muscles were active in the same order as the muscle groups are recruited during crawling behavior. A third fictive motor pattern exhibits a left-right asymmetry across segments and bears similarities with turning behavior in intact larvae, occurring equally frequently and involving asymmetry in the same segments. Ablation of the segments in which forward and backward waves of Ca2+ signals were normally initiated did not eliminate production of Ca2+ waves. When the brain and subesophageal ganglion (SOG) were removed, the remaining ganglia retained the ability to produce both forward and backward waves of motor activity, although the speed and frequency of waves changed. Bilateral asymmetry of activity was reduced when the brain was removed and abolished when the SOG was removed. This work paves the way to studying the neural and genetic underpinnings of segmentally coordinated motor pattern generation in Drosophila with imaging techniques.
publisher	American Physiological Society
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637366/
_version_	1613498956575145984

Imaging fictive locomotor patterns in larval Drosophila

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