RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK

Glioblastoma (GBM) are characterized by increased invasion into the surrounding normal brain tissue. RTVP-1 is highly expressed in GBM and regulates the migration and invasion of glioma cells. To further study RTVP-1 effects we performed a pull-down assay using His-tagged RTVP-1 followed by mass spe...

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Main Authors:	Ziv-Av, Amotz, Giladi, Nissim David, Lee, Hae Kyung, Cazacu, Simona, Finniss, Susan, Xiang, Cunli, Pauker, Maor H., Barda-Saad, Mira, Poisson, Laila, Brodie, Chaya
Format:	Online
Language:	English
Published:	Impact Journals LLC 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637324/

id	pubmed-4637324
recordtype	oai_dc
spelling	pubmed-46373242015-12-02 RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK Ziv-Av, Amotz Giladi, Nissim David Lee, Hae Kyung Cazacu, Simona Finniss, Susan Xiang, Cunli Pauker, Maor H. Barda-Saad, Mira Poisson, Laila Brodie, Chaya Research Paper Glioblastoma (GBM) are characterized by increased invasion into the surrounding normal brain tissue. RTVP-1 is highly expressed in GBM and regulates the migration and invasion of glioma cells. To further study RTVP-1 effects we performed a pull-down assay using His-tagged RTVP-1 followed by mass spectrometry and found that RTVP-1 was associated with the actin polymerization regulator, N-WASP. This association was further validated by co-immunoprecipitation and FRET analysis. We found that RTVP-1 increased cell spreading, migration and invasion and these effects were at least partly mediated by N-WASP. Another protein which was found by the pull-down assay to interact with RTVP-1 is hnRNPK. This protein has been recently reported to associate with and to inhibit the effect of N-WASP on cell spreading. hnRNPK decreased cell migration, spreading and invasion in glioma cells. Using co-immunoprecipitation we validated the interactions of hnRNPK with N-WASP and RTVP-1 in glioma cells. In addition, we found that overexpression of RTVP-1 decreased the association of N-WASP and hnRNPK. In summary, we report that RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein. Impact Journals LLC 2015-06-15 /pmc/articles/PMC4637324/ /pubmed/26305187 Text en Copyright: © 2015 Ziv-Av et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ziv-Av, Amotz Giladi, Nissim David Lee, Hae Kyung Cazacu, Simona Finniss, Susan Xiang, Cunli Pauker, Maor H. Barda-Saad, Mira Poisson, Laila Brodie, Chaya
spellingShingle	Ziv-Av, Amotz Giladi, Nissim David Lee, Hae Kyung Cazacu, Simona Finniss, Susan Xiang, Cunli Pauker, Maor H. Barda-Saad, Mira Poisson, Laila Brodie, Chaya RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK
author_facet	Ziv-Av, Amotz Giladi, Nissim David Lee, Hae Kyung Cazacu, Simona Finniss, Susan Xiang, Cunli Pauker, Maor H. Barda-Saad, Mira Poisson, Laila Brodie, Chaya
author_sort	Ziv-Av, Amotz
title	RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK
title_short	RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK
title_full	RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK
title_fullStr	RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK
title_full_unstemmed	RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK
title_sort	rtvp-1 regulates glioma cell migration and invasion via interaction with n-wasp and hnrnpk
description	Glioblastoma (GBM) are characterized by increased invasion into the surrounding normal brain tissue. RTVP-1 is highly expressed in GBM and regulates the migration and invasion of glioma cells. To further study RTVP-1 effects we performed a pull-down assay using His-tagged RTVP-1 followed by mass spectrometry and found that RTVP-1 was associated with the actin polymerization regulator, N-WASP. This association was further validated by co-immunoprecipitation and FRET analysis. We found that RTVP-1 increased cell spreading, migration and invasion and these effects were at least partly mediated by N-WASP. Another protein which was found by the pull-down assay to interact with RTVP-1 is hnRNPK. This protein has been recently reported to associate with and to inhibit the effect of N-WASP on cell spreading. hnRNPK decreased cell migration, spreading and invasion in glioma cells. Using co-immunoprecipitation we validated the interactions of hnRNPK with N-WASP and RTVP-1 in glioma cells. In addition, we found that overexpression of RTVP-1 decreased the association of N-WASP and hnRNPK. In summary, we report that RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein.
publisher	Impact Journals LLC
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637324/
_version_	1613498933292564480

RTVP-1 regulates glioma cell migration and invasion via interaction with N-WASP and hnRNPK

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