An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food

Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food tes...

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Main Authors:	Law, Jodi Woan-Fei, Ab Mutalib, Nurul-Syakima, Chan, Kok-Gan, Lee, Learn-Han
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630303/

id	pubmed-4630303
recordtype	oai_dc
spelling	pubmed-46303032015-11-17 An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food Law, Jodi Woan-Fei Ab Mutalib, Nurul-Syakima Chan, Kok-Gan Lee, Learn-Han Microbiology Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration, and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria enrichment broth, Fraser broth, and University of Vermont Medium (UVM) Listeria enrichment broth are recommended by regulatory agencies such as Food and Drug Administration-bacteriological and analytical method (FDA-BAM), US Department of Agriculture-Food and Safety (USDA-FSIS), and International Organization for Standardization (ISO). Many plating media are available for the isolation of L. monocytogenes, for instance, polymyxin acriflavin lithium-chloride ceftazidime aesculin mannitol, Oxford, and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method, and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. most probable number technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction, multiplex polymerase chain reaction, real-time/quantitative polymerase chain reaction, nucleic acid sequence-based amplification, loop-mediated isothermal amplification, DNA microarray, and next generation sequencing technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labor-saving. In future, there are chances for the development of new techniques for the detection and identification of foodborne with improved features. Frontiers Media S.A. 2015-11-03 /pmc/articles/PMC4630303/ /pubmed/26579116 http://dx.doi.org/10.3389/fmicb.2015.01227 Text en Copyright © 2015 Law, Ab Mutalib, Chan and Lee. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Law, Jodi Woan-Fei Ab Mutalib, Nurul-Syakima Chan, Kok-Gan Lee, Learn-Han
spellingShingle	Law, Jodi Woan-Fei Ab Mutalib, Nurul-Syakima Chan, Kok-Gan Lee, Learn-Han An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
author_facet	Law, Jodi Woan-Fei Ab Mutalib, Nurul-Syakima Chan, Kok-Gan Lee, Learn-Han
author_sort	Law, Jodi Woan-Fei
title	An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
title_short	An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
title_full	An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
title_fullStr	An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
title_full_unstemmed	An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
title_sort	insight into the isolation, enumeration, and molecular detection of listeria monocytogenes in food
description	Listeria monocytogenes, a foodborne pathogen that can cause listeriosis through the consumption of food contaminated with this pathogen. The ability of L. monocytogenes to survive in extreme conditions and cause food contaminations have become a major concern. Hence, routine microbiological food testing is necessary to prevent food contamination and outbreaks of foodborne illness. This review provides insight into the methods for cultural detection, enumeration, and molecular identification of L. monocytogenes in various food samples. There are a number of enrichment and plating media that can be used for the isolation of L. monocytogenes from food samples. Enrichment media such as buffered Listeria enrichment broth, Fraser broth, and University of Vermont Medium (UVM) Listeria enrichment broth are recommended by regulatory agencies such as Food and Drug Administration-bacteriological and analytical method (FDA-BAM), US Department of Agriculture-Food and Safety (USDA-FSIS), and International Organization for Standardization (ISO). Many plating media are available for the isolation of L. monocytogenes, for instance, polymyxin acriflavin lithium-chloride ceftazidime aesculin mannitol, Oxford, and other chromogenic media. Besides, reference methods like FDA-BAM, ISO 11290 method, and USDA-FSIS method are usually applied for the cultural detection or enumeration of L. monocytogenes. most probable number technique is applied for the enumeration of L. monocytogenes in the case of low level contamination. Molecular methods including polymerase chain reaction, multiplex polymerase chain reaction, real-time/quantitative polymerase chain reaction, nucleic acid sequence-based amplification, loop-mediated isothermal amplification, DNA microarray, and next generation sequencing technology for the detection and identification of L. monocytogenes are discussed in this review. Overall, molecular methods are rapid, sensitive, specific, time- and labor-saving. In future, there are chances for the development of new techniques for the detection and identification of foodborne with improved features.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630303/
_version_	1613496303974612992

An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food

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