Characterization of impurities in cefpodoxime proxetil using LC–MSn
Reversed-phase liquid chromatography coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS) was used to characterize impurities in cefpodoxime proxetil, an ester-modified prodrug. Based on the mechanisms by which cephalosporins are degraded, stress tests were designed and performe...
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| Format: | Online |
| Language: | English |
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Elsevier
2014
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629086/ |
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pubmed-46290862015-11-17 Characterization of impurities in cefpodoxime proxetil using LC–MSn Li, Jin Zhang, Dousheng Hu, Changqin Original Article Reversed-phase liquid chromatography coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS) was used to characterize impurities in cefpodoxime proxetil, an ester-modified prodrug. Based on the mechanisms by which cephalosporins are degraded, stress tests were designed and performed. The bulk material and capsule were eluted through a C18 column with formic acid–methanol–water as the mobile phase. In total, 15 impurities were characterized in commercial samples, including 7 known impurities and 8 new impurities. The structures of these unknown compounds were deduced via comparison with the fragmentation patterns of cefpodoxime proxetil. Data from this systematic study will help improve the safety and quality of cefpodoxime proxetil. Elsevier 2014-08 2014-07-14 /pmc/articles/PMC4629086/ /pubmed/26579402 http://dx.doi.org/10.1016/j.apsb.2014.06.007 Text en © 2014 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Li, Jin Zhang, Dousheng Hu, Changqin |
| spellingShingle |
Li, Jin Zhang, Dousheng Hu, Changqin Characterization of impurities in cefpodoxime proxetil using LC–MSn |
| author_facet |
Li, Jin Zhang, Dousheng Hu, Changqin |
| author_sort |
Li, Jin |
| title |
Characterization of impurities in cefpodoxime proxetil using LC–MSn |
| title_short |
Characterization of impurities in cefpodoxime proxetil using LC–MSn |
| title_full |
Characterization of impurities in cefpodoxime proxetil using LC–MSn |
| title_fullStr |
Characterization of impurities in cefpodoxime proxetil using LC–MSn |
| title_full_unstemmed |
Characterization of impurities in cefpodoxime proxetil using LC–MSn |
| title_sort |
characterization of impurities in cefpodoxime proxetil using lc–msn |
| description |
Reversed-phase liquid chromatography coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS) was used to characterize impurities in cefpodoxime proxetil, an ester-modified prodrug. Based on the mechanisms by which cephalosporins are degraded, stress tests were designed and performed. The bulk material and capsule were eluted through a C18 column with formic acid–methanol–water as the mobile phase. In total, 15 impurities were characterized in commercial samples, including 7 known impurities and 8 new impurities. The structures of these unknown compounds were deduced via comparison with the fragmentation patterns of cefpodoxime proxetil. Data from this systematic study will help improve the safety and quality of cefpodoxime proxetil. |
| publisher |
Elsevier |
| publishDate |
2014 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4629086/ |
| _version_ |
1613495943654539264 |