SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells

LSD1 is a histone lysine demethylase, which is highly expressed in multiple types of human cancer. Although its roles in transcriptional regulation have been well-studied, functional regulation of LSD1 by post-translational modifications still remains unknown. Here, we demonstrate that the histone l...

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Main Authors:	Piao, Lianhua, Suzuki, Takehiro, Dohmae, Naoshi, Nakamura, Yusuke, Hamamoto, Ryuji
Format:	Online
Language:	English
Published:	Impact Journals LLC 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627283/

id	pubmed-4627283
recordtype	oai_dc
spelling	pubmed-46272832015-12-02 SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells Piao, Lianhua Suzuki, Takehiro Dohmae, Naoshi Nakamura, Yusuke Hamamoto, Ryuji Priority Research Paper LSD1 is a histone lysine demethylase, which is highly expressed in multiple types of human cancer. Although its roles in transcriptional regulation have been well-studied, functional regulation of LSD1 by post-translational modifications still remains unknown. Here, we demonstrate that the histone lysine methyltransferase SUV39H2 trimethylated LSD1 on lysine 322. Knockdown of SUV39H2 resulted in a decrease of LSD1 protein even though the mRNA levels were unchanged. SUV39H2-induced LSD1 methylation suppresses LSD1 polyubiquitination and subsequent degradation. In addition, we also observed indirect effect of SUV39H2 overexpression on LSD1-target genes. Our results reveal the regulatory mechanism of LSD1 protein through its lysine methylation by SUV39H2 in human cancer cells. Impact Journals LLC 2015-07-03 /pmc/articles/PMC4627283/ /pubmed/26183527 Text en Copyright: © 2015 Piao et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Piao, Lianhua Suzuki, Takehiro Dohmae, Naoshi Nakamura, Yusuke Hamamoto, Ryuji
spellingShingle	Piao, Lianhua Suzuki, Takehiro Dohmae, Naoshi Nakamura, Yusuke Hamamoto, Ryuji SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells
author_facet	Piao, Lianhua Suzuki, Takehiro Dohmae, Naoshi Nakamura, Yusuke Hamamoto, Ryuji
author_sort	Piao, Lianhua
title	SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells
title_short	SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells
title_full	SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells
title_fullStr	SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells
title_full_unstemmed	SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells
title_sort	suv39h2 methylates and stabilizes lsd1 by inhibiting polyubiquitination in human cancer cells
description	LSD1 is a histone lysine demethylase, which is highly expressed in multiple types of human cancer. Although its roles in transcriptional regulation have been well-studied, functional regulation of LSD1 by post-translational modifications still remains unknown. Here, we demonstrate that the histone lysine methyltransferase SUV39H2 trimethylated LSD1 on lysine 322. Knockdown of SUV39H2 resulted in a decrease of LSD1 protein even though the mRNA levels were unchanged. SUV39H2-induced LSD1 methylation suppresses LSD1 polyubiquitination and subsequent degradation. In addition, we also observed indirect effect of SUV39H2 overexpression on LSD1-target genes. Our results reveal the regulatory mechanism of LSD1 protein through its lysine methylation by SUV39H2 in human cancer cells.
publisher	Impact Journals LLC
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4627283/
_version_	1613495319803199488

SUV39H2 methylates and stabilizes LSD1 by inhibiting polyubiquitination in human cancer cells

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