Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells

Quercetin is a dietary flavonoid which exerts anti-oxidant, anti-inflammatory and anti-cancer properties. In this study, we investigated the anti-proliferative effect of quercetin in two breast cancer cell lines (MCF-7 and MDA-MB-231), which differed in hormone receptor. IC50 value (37μM) of quercet...

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Main Authors:	Ranganathan, Santhalakshmi, Halagowder, Devaraj, Sivasithambaram, Niranjali Devaraj
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619597/

id	pubmed-4619597
recordtype	oai_dc
spelling	pubmed-46195972015-10-29 Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells Ranganathan, Santhalakshmi Halagowder, Devaraj Sivasithambaram, Niranjali Devaraj Research Article Quercetin is a dietary flavonoid which exerts anti-oxidant, anti-inflammatory and anti-cancer properties. In this study, we investigated the anti-proliferative effect of quercetin in two breast cancer cell lines (MCF-7 and MDA-MB-231), which differed in hormone receptor. IC50 value (37μM) of quercetin showed significant cytotoxicity in MCF-7 cells, which was not observed in MDA-MB-231 cells even at 100μM of quercetin treatment. To study the response of cancer cells to quercetin, with respect to different hormone receptors, both the cell lines were treated with a fixed concentration (40μM) of quercetin. MCF-7 cells on quercetin treatment showed more apoptotic cells with G1 phase arrest. In addition, quercetin effectively suppressed the expression of CyclinD1, p21, Twist and phospho p38MAPK, which was not observed in MDA-MB-231 cells. To analyse the molecular mechanism of quercetin in exerting an apoptotic effect in MCF-7 cells, Twist was over-expressed and the molecular changes were observed after quercetin administration. Quercetin effectively regulated the expression of Twist, in turn p16 and p21 which induced apoptosis in MCF-7 cells. In conclusion, quercetin induces apoptosis in breast cancer cells through suppression of Twist via p38MAPK pathway. Public Library of Science 2015-10-22 /pmc/articles/PMC4619597/ /pubmed/26491966 http://dx.doi.org/10.1371/journal.pone.0141370 Text en © 2015 Ranganathan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ranganathan, Santhalakshmi Halagowder, Devaraj Sivasithambaram, Niranjali Devaraj
spellingShingle	Ranganathan, Santhalakshmi Halagowder, Devaraj Sivasithambaram, Niranjali Devaraj Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
author_facet	Ranganathan, Santhalakshmi Halagowder, Devaraj Sivasithambaram, Niranjali Devaraj
author_sort	Ranganathan, Santhalakshmi
title	Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_short	Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_full	Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_fullStr	Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_full_unstemmed	Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_sort	quercetin suppresses twist to induce apoptosis in mcf-7 breast cancer cells
description	Quercetin is a dietary flavonoid which exerts anti-oxidant, anti-inflammatory and anti-cancer properties. In this study, we investigated the anti-proliferative effect of quercetin in two breast cancer cell lines (MCF-7 and MDA-MB-231), which differed in hormone receptor. IC50 value (37μM) of quercetin showed significant cytotoxicity in MCF-7 cells, which was not observed in MDA-MB-231 cells even at 100μM of quercetin treatment. To study the response of cancer cells to quercetin, with respect to different hormone receptors, both the cell lines were treated with a fixed concentration (40μM) of quercetin. MCF-7 cells on quercetin treatment showed more apoptotic cells with G1 phase arrest. In addition, quercetin effectively suppressed the expression of CyclinD1, p21, Twist and phospho p38MAPK, which was not observed in MDA-MB-231 cells. To analyse the molecular mechanism of quercetin in exerting an apoptotic effect in MCF-7 cells, Twist was over-expressed and the molecular changes were observed after quercetin administration. Quercetin effectively regulated the expression of Twist, in turn p16 and p21 which induced apoptosis in MCF-7 cells. In conclusion, quercetin induces apoptosis in breast cancer cells through suppression of Twist via p38MAPK pathway.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619597/
_version_	1613492661331689472

Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells

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