The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells

Doppel (Dpl) protein is the paralogue of the cellular prion (PrP) protein. In humans, Dpl is expressed almost exclusively in testis where it is involved in spermatogenesis. Recently, the protein has been described to be ectopically expressed in astrocytomas and its potential association to the brain...

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Main Authors:	Azzalin, Alberto, Sbalchiero, Elena, Barbieri, Giulia, Palumbo, Silvia, Muzzini, Cristina, Comincini, Sergio
Format:	Online
Language:	English
Published:	IOS Press 2008
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618816/

id	pubmed-4618816
recordtype	oai_dc
spelling	pubmed-46188162016-01-12 The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells Azzalin, Alberto Sbalchiero, Elena Barbieri, Giulia Palumbo, Silvia Muzzini, Cristina Comincini, Sergio Other Doppel (Dpl) protein is the paralogue of the cellular prion (PrP) protein. In humans, Dpl is expressed almost exclusively in testis where it is involved in spermatogenesis. Recently, the protein has been described to be ectopically expressed in astrocytomas and its potential association to the brain tumor malignancy progression has been advanced. In this study, we aimed to investigate in vitro the potential involvement of Dpl in the tumor cell migration: to this purpose, Dpl expression was reduced in the IPDDC-A2 astrocytoma-derived cell line, by means of antisense and siRNA approaches; migration rates were then evaluated by means of a scratch wound healing assay. As a result, the cellular migration was sensibly reduced after Dpl silencing. Following a complementary approach, in HeLa cells, showing very low endogenous Dpl expression, the protein expression was induced by transfection and stabilization of an eukaryotic expression vector containing the doppel gene coding sequence. These stably Dpl-overexpressing cells revealed a significant increase in the migration rate, compared to untreated and control cells. In addition, Dpl-forced expression induced substantial changes in the cell morphology. Of note, in these cells, viability examination by means of tetrazolium-based assay did not reveal differences in the proliferation; on the contrary, a variation in density-dependent growth, leading to an increase of cell contact inhibition was highlighted. These results, in conclusion, might suggest a potential and functional role for Dpl in tumor cells migratory and morphological behaviours and address to future gene-targeted therapeutic interventions. IOS Press 2008 2008-10-14 /pmc/articles/PMC4618816/ /pubmed/18936526 http://dx.doi.org/10.3233/CLO-2008-0437 Text en Copyright © 2008 Hindawi Publishing Corporation and the authors.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Azzalin, Alberto Sbalchiero, Elena Barbieri, Giulia Palumbo, Silvia Muzzini, Cristina Comincini, Sergio
spellingShingle	Azzalin, Alberto Sbalchiero, Elena Barbieri, Giulia Palumbo, Silvia Muzzini, Cristina Comincini, Sergio The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells
author_facet	Azzalin, Alberto Sbalchiero, Elena Barbieri, Giulia Palumbo, Silvia Muzzini, Cristina Comincini, Sergio
author_sort	Azzalin, Alberto
title	The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells
title_short	The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells
title_full	The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells
title_fullStr	The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells
title_full_unstemmed	The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells
title_sort	doppel (dpl) protein influences in vitro migration capability in astrocytoma-derived cells
description	Doppel (Dpl) protein is the paralogue of the cellular prion (PrP) protein. In humans, Dpl is expressed almost exclusively in testis where it is involved in spermatogenesis. Recently, the protein has been described to be ectopically expressed in astrocytomas and its potential association to the brain tumor malignancy progression has been advanced. In this study, we aimed to investigate in vitro the potential involvement of Dpl in the tumor cell migration: to this purpose, Dpl expression was reduced in the IPDDC-A2 astrocytoma-derived cell line, by means of antisense and siRNA approaches; migration rates were then evaluated by means of a scratch wound healing assay. As a result, the cellular migration was sensibly reduced after Dpl silencing. Following a complementary approach, in HeLa cells, showing very low endogenous Dpl expression, the protein expression was induced by transfection and stabilization of an eukaryotic expression vector containing the doppel gene coding sequence. These stably Dpl-overexpressing cells revealed a significant increase in the migration rate, compared to untreated and control cells. In addition, Dpl-forced expression induced substantial changes in the cell morphology. Of note, in these cells, viability examination by means of tetrazolium-based assay did not reveal differences in the proliferation; on the contrary, a variation in density-dependent growth, leading to an increase of cell contact inhibition was highlighted. These results, in conclusion, might suggest a potential and functional role for Dpl in tumor cells migratory and morphological behaviours and address to future gene-targeted therapeutic interventions.
publisher	IOS Press
publishDate	2008
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618816/
_version_	1613492271853862912

The doppel (Dpl) Protein Influences In Vitro Migration Capability in Astrocytoma-Derived Cells

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