Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples

The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed...

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Main Authors:	Krüger, Alejandra, Lucchesi, Paula M. A., Sanso, A. Mariel, Etcheverría, Analía I., Bustamante, Ana V., Burgán, Julia, Fernández, Luciana, Fernández, Daniel, Leotta, Gerardo, Friedrich, Alexander W., Padola, Nora L., Rossen, John W. A.
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/

id	pubmed-4612136
recordtype	oai_dc
spelling	pubmed-46121362015-11-04 Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A. Microbiology The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a. Frontiers Media S.A. 2015-10-20 /pmc/articles/PMC4612136/ /pubmed/26539413 http://dx.doi.org/10.3389/fcimb.2015.00074 Text en Copyright © 2015 Krüger, Lucchesi, Sanso, Etcheverría, Bustamante, Burgán, Fernández, Fernández, Leotta, Friedrich, Padola and Rossen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A.
spellingShingle	Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A. Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
author_facet	Krüger, Alejandra Lucchesi, Paula M. A. Sanso, A. Mariel Etcheverría, Analía I. Bustamante, Ana V. Burgán, Julia Fernández, Luciana Fernández, Daniel Leotta, Gerardo Friedrich, Alexander W. Padola, Nora L. Rossen, John W. A.
author_sort	Krüger, Alejandra
title	Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_short	Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_full	Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_fullStr	Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_full_unstemmed	Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples
title_sort	genetic characterization of shiga toxin-producing escherichia coli o26:h11 strains isolated from animal, food, and clinical samples
description	The Shiga-toxin producing Escherichia coli (STEC) may cause serious illness in human. Here we analyze O26:H11 strains known to be among the most reported STEC strains causing human infections. Genetic characterization of strains isolated from animal, food, and clinical specimens in Argentina showed that most carried either stx1a or stx2a subtypes. Interestingly, stx2a-positive O26:H11 rarely isolated from cattle in other countries showed to be an important proportion of O26:H11 strains circulating in cattle and food in our region. Seventeen percent of the isolates harbored more than one gene associated with antimicrobial resistance. In addition to stx, all strains contained the virulence genes eae-β, tir, efa, iha, espB, cif, espA, espF, espJ, nleA, nleB, nleC, and iss; and all except one contained ehxA, espP, and cba genes. On the other hand, toxB and espI genes were exclusively observed in stx2-positive isolates, whereas katP was only found in stx1a-positive isolates. Our results show that O26:H11 STEC strains circulating in Argentina, including those isolated from humans, cattle, and meat products, present a high pathogenic potential, and evidence that cattle can be a reservoir of O26:H11 strains harboring stx2a.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612136/
_version_	1613490275435413504

Genetic characterization of Shiga toxin-producing Escherichia coli O26:H11 strains isolated from animal, food, and clinical samples

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