Loop Mediated Isothermal Amplification for Detection of Trypanosoma brucei gambiense in Urine and Saliva Samples in Nonhuman Primate Model
Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease. The disease caused by Trypanosoma brucei gambiense is the most prevalent in Africa. Early diagnosis is hampered by lack of sensitive diagnostic techniques. This study explored the potential of loop mediated isothermal...
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Hindawi Publishing Corporation
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609394/ |
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pubmed-46093942015-10-26 Loop Mediated Isothermal Amplification for Detection of Trypanosoma brucei gambiense in Urine and Saliva Samples in Nonhuman Primate Model Ngotho, Maina Kagira, John Maina Gachie, Beatrice Muthoni Karanja, Simon Muturi Waema, Maxwell Wambua Maranga, Dawn Nyawira Maina, Naomi Wangari Research Article Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease. The disease caused by Trypanosoma brucei gambiense is the most prevalent in Africa. Early diagnosis is hampered by lack of sensitive diagnostic techniques. This study explored the potential of loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) in the detection of T. b. gambiense infection in a vervet monkey HAT model. Six vervet monkeys were experimentally infected with T. b. gambiense IL3253 and monitored for 180 days after infection. Parasitaemia was scored daily. Blood, cerebrospinal fluid (CSF), saliva, and urine samples were collected weekly. PCR and LAMP were performed on serum, CSF, saliva, and urine samples. The detection by LAMP was significantly higher than that of parasitological methods and PCR in all the samples. The performance of LAMP varied between the samples and was better in serum followed by saliva and then urine samples. In the saliva samples, LAMP had 100% detection between 21 and 77 dpi, whereas in urine the detection it was slightly lower, but there was over 80% detection between 28 and 91 dpi. However, LAMP could not detect trypanosomes in either saliva or urine after 140 and 126 dpi, respectively. The findings of this study emphasize the importance of LAMP in diagnosis of HAT using saliva and urine samples. Hindawi Publishing Corporation 2015 2015-10-04 /pmc/articles/PMC4609394/ /pubmed/26504841 http://dx.doi.org/10.1155/2015/867846 Text en Copyright © 2015 Maina Ngotho et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ngotho, Maina Kagira, John Maina Gachie, Beatrice Muthoni Karanja, Simon Muturi Waema, Maxwell Wambua Maranga, Dawn Nyawira Maina, Naomi Wangari |
| spellingShingle |
Ngotho, Maina Kagira, John Maina Gachie, Beatrice Muthoni Karanja, Simon Muturi Waema, Maxwell Wambua Maranga, Dawn Nyawira Maina, Naomi Wangari Loop Mediated Isothermal Amplification for Detection of Trypanosoma brucei gambiense in Urine and Saliva Samples in Nonhuman Primate Model |
| author_facet |
Ngotho, Maina Kagira, John Maina Gachie, Beatrice Muthoni Karanja, Simon Muturi Waema, Maxwell Wambua Maranga, Dawn Nyawira Maina, Naomi Wangari |
| author_sort |
Ngotho, Maina |
| title |
Loop Mediated Isothermal Amplification for Detection of
Trypanosoma brucei gambiense in Urine and Saliva Samples in
Nonhuman Primate Model |
| title_short |
Loop Mediated Isothermal Amplification for Detection of
Trypanosoma brucei gambiense in Urine and Saliva Samples in
Nonhuman Primate Model |
| title_full |
Loop Mediated Isothermal Amplification for Detection of
Trypanosoma brucei gambiense in Urine and Saliva Samples in
Nonhuman Primate Model |
| title_fullStr |
Loop Mediated Isothermal Amplification for Detection of
Trypanosoma brucei gambiense in Urine and Saliva Samples in
Nonhuman Primate Model |
| title_full_unstemmed |
Loop Mediated Isothermal Amplification for Detection of
Trypanosoma brucei gambiense in Urine and Saliva Samples in
Nonhuman Primate Model |
| title_sort |
loop mediated isothermal amplification for detection of
trypanosoma brucei gambiense in urine and saliva samples in
nonhuman primate model |
| description |
Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease. The disease caused by Trypanosoma brucei gambiense is the most prevalent in Africa. Early diagnosis is hampered by lack of sensitive diagnostic techniques. This study explored the potential of loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) in the detection of T. b. gambiense infection in a vervet monkey HAT model. Six vervet monkeys were experimentally infected with T. b. gambiense IL3253 and monitored for 180 days after infection. Parasitaemia was scored daily. Blood, cerebrospinal fluid (CSF), saliva, and urine samples were collected weekly. PCR and LAMP were performed on serum, CSF, saliva, and urine samples. The detection by LAMP was significantly higher than that of parasitological methods and PCR in all the samples. The performance of LAMP varied between the samples and was better in serum followed by saliva and then urine samples. In the saliva samples, LAMP had 100% detection between 21 and 77 dpi, whereas in urine the detection it was slightly lower, but there was over 80% detection between 28 and 91 dpi. However, LAMP could not detect trypanosomes in either saliva or urine after 140 and 126 dpi, respectively. The findings of this study emphasize the importance of LAMP in diagnosis of HAT using saliva and urine samples. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4609394/ |
| _version_ |
1613489480042283008 |