Inhibition of LDHA suppresses tumor progression in prostate cancer

A key hallmark of cancer cells is their altered metabolism, known as Warburg effect. Lactate dehydrogenase A (LDHA) executes the final step of aerobic glycolysis and has been reported to be involved in the tumor progression. However, the function of LDHA in prostate cancer has not been studied. In c...

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Main Authors:	Xian, Zhi-Yong, Liu, Jiu-Min, Chen, Qing-Ke, Chen, Han-Zhong, Ye, Chu-Jin, Xue, Jian, Yang, Huan-Qing, Li, Jing-Lei, Liu, Xue-Feng, Kuang, Su-Juan
Format:	Online
Language:	English
Published:	Springer Netherlands 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605959/

id	pubmed-4605959
recordtype	oai_dc
spelling	pubmed-46059592015-10-19 Inhibition of LDHA suppresses tumor progression in prostate cancer Xian, Zhi-Yong Liu, Jiu-Min Chen, Qing-Ke Chen, Han-Zhong Ye, Chu-Jin Xue, Jian Yang, Huan-Qing Li, Jing-Lei Liu, Xue-Feng Kuang, Su-Juan Research Article A key hallmark of cancer cells is their altered metabolism, known as Warburg effect. Lactate dehydrogenase A (LDHA) executes the final step of aerobic glycolysis and has been reported to be involved in the tumor progression. However, the function of LDHA in prostate cancer has not been studied. In current study, we observed overexpression of LDHA in the clinical prostate cancer samples compared with benign prostate hyperplasia tissues as demonstrated by immunohistochemistry and real-time qPCR. Attenuated expression of LDHA by siRNA or inhibition of LDHA activities by FX11 inhibited cell proliferation, migration, invasion, and promoted cell apoptosis of PC-3 and DU145 cells. Mechanistically, decreased Warburg effect as demonstrated by reduced glucose consumption and lactate secretion and reduced expression of MMP-9, PLAU, and cathepsin B were found after LDHA knockdown or FX11 treatment in PC-3 and DU145 cells. Taken together, our study revealed the oncogenic role of LDHA in prostate cancer and suggested that LDHA might be a potential therapeutic target. Springer Netherlands 2015-05-16 /pmc/articles/PMC4605959/ /pubmed/25983002 http://dx.doi.org/10.1007/s13277-015-3540-x Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Xian, Zhi-Yong Liu, Jiu-Min Chen, Qing-Ke Chen, Han-Zhong Ye, Chu-Jin Xue, Jian Yang, Huan-Qing Li, Jing-Lei Liu, Xue-Feng Kuang, Su-Juan
spellingShingle	Xian, Zhi-Yong Liu, Jiu-Min Chen, Qing-Ke Chen, Han-Zhong Ye, Chu-Jin Xue, Jian Yang, Huan-Qing Li, Jing-Lei Liu, Xue-Feng Kuang, Su-Juan Inhibition of LDHA suppresses tumor progression in prostate cancer
author_facet	Xian, Zhi-Yong Liu, Jiu-Min Chen, Qing-Ke Chen, Han-Zhong Ye, Chu-Jin Xue, Jian Yang, Huan-Qing Li, Jing-Lei Liu, Xue-Feng Kuang, Su-Juan
author_sort	Xian, Zhi-Yong
title	Inhibition of LDHA suppresses tumor progression in prostate cancer
title_short	Inhibition of LDHA suppresses tumor progression in prostate cancer
title_full	Inhibition of LDHA suppresses tumor progression in prostate cancer
title_fullStr	Inhibition of LDHA suppresses tumor progression in prostate cancer
title_full_unstemmed	Inhibition of LDHA suppresses tumor progression in prostate cancer
title_sort	inhibition of ldha suppresses tumor progression in prostate cancer
description	A key hallmark of cancer cells is their altered metabolism, known as Warburg effect. Lactate dehydrogenase A (LDHA) executes the final step of aerobic glycolysis and has been reported to be involved in the tumor progression. However, the function of LDHA in prostate cancer has not been studied. In current study, we observed overexpression of LDHA in the clinical prostate cancer samples compared with benign prostate hyperplasia tissues as demonstrated by immunohistochemistry and real-time qPCR. Attenuated expression of LDHA by siRNA or inhibition of LDHA activities by FX11 inhibited cell proliferation, migration, invasion, and promoted cell apoptosis of PC-3 and DU145 cells. Mechanistically, decreased Warburg effect as demonstrated by reduced glucose consumption and lactate secretion and reduced expression of MMP-9, PLAU, and cathepsin B were found after LDHA knockdown or FX11 treatment in PC-3 and DU145 cells. Taken together, our study revealed the oncogenic role of LDHA in prostate cancer and suggested that LDHA might be a potential therapeutic target.
publisher	Springer Netherlands
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4605959/
_version_	1613488233089335296

Inhibition of LDHA suppresses tumor progression in prostate cancer

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