BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis

Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol–cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better un...

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Main Authors:	Liu, Tongkun, Qian, Yu, Duan, Weike, Ren, Jun, Hou, Xilin, Li, Ying
Format:	Online
Language:	English
Published:	Nature Publishing Group 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596333/

id	pubmed-4596333
recordtype	oai_dc
spelling	pubmed-45963332015-10-26 BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis Liu, Tongkun Qian, Yu Duan, Weike Ren, Jun Hou, Xilin Li, Ying Article Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol–cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better understand the biological function of the ubiquinol–cytochrome c reductase iron–sulfur protein, the full-length cDNA of BcRISP1 was cloned; it was found to contain 810 base pairs and encode 269 amino acids. Unusually, high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of cytoplasmic male sterile lines and maintainer lines. The seed set was affected by the overexpression of BcRISP1, and shorter siliques with lower seed sets were observed in 35S::BcRISP1 Arabidopsis plants. These characteristics may have resulted from the reduced formation of pollen and impaired pollen tube growth. qRT-PCR results revealed that in 35S::BcRISP1 plants, the expression levels of the mitochondrial respiratory chain-related genes, COX10 and RIP1, were enhanced, whereas the expression levels of QCR7 and SDH2-1 were reduced. This result implies that overexpression of BcRISP1 in transgenic Arabidopsis plants may disrupt the mitochondrial electron transport chain by affecting the expression of mitochondrial respiratory chain-related genes and therefore, reducing the seed set. Nature Publishing Group 2014-12-10 /pmc/articles/PMC4596333/ /pubmed/26504557 http://dx.doi.org/10.1038/hortres.2014.62 Text en Copyright © 2014 Nanjing Agricultural University http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liu, Tongkun Qian, Yu Duan, Weike Ren, Jun Hou, Xilin Li, Ying
spellingShingle	Liu, Tongkun Qian, Yu Duan, Weike Ren, Jun Hou, Xilin Li, Ying BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
author_facet	Liu, Tongkun Qian, Yu Duan, Weike Ren, Jun Hou, Xilin Li, Ying
author_sort	Liu, Tongkun
title	BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
title_short	BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
title_full	BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
title_fullStr	BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
title_full_unstemmed	BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
title_sort	bcrisp1, isolated from non-heading chinese cabbage, decreases the seed set of transgenic arabidopsis
description	Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol–cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better understand the biological function of the ubiquinol–cytochrome c reductase iron–sulfur protein, the full-length cDNA of BcRISP1 was cloned; it was found to contain 810 base pairs and encode 269 amino acids. Unusually, high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of cytoplasmic male sterile lines and maintainer lines. The seed set was affected by the overexpression of BcRISP1, and shorter siliques with lower seed sets were observed in 35S::BcRISP1 Arabidopsis plants. These characteristics may have resulted from the reduced formation of pollen and impaired pollen tube growth. qRT-PCR results revealed that in 35S::BcRISP1 plants, the expression levels of the mitochondrial respiratory chain-related genes, COX10 and RIP1, were enhanced, whereas the expression levels of QCR7 and SDH2-1 were reduced. This result implies that overexpression of BcRISP1 in transgenic Arabidopsis plants may disrupt the mitochondrial electron transport chain by affecting the expression of mitochondrial respiratory chain-related genes and therefore, reducing the seed set.
publisher	Nature Publishing Group
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4596333/
_version_	1613484547196846080

BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis

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