DNA Ligase I Is Not Essential for Mammalian Cell Viability

DNA Ligase I Is Not Essential for Mammalian Cell Viability

Of the three DNA ligases present in all vertebrates, DNA ligase I (Lig1) has been considered essential for ligating Okazaki fragments during DNA replication and thereby essential for cell viability. Here, we report the striking finding that a Lig1-null murine B cell line is viable. Surprisingly, the...

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Main Authors: Han, Li, Masani, Shahnaz, Hsieh, Chih-lin, Yu, Kefei
Format: Online
Language:English
Published: 2014
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593317/
id pubmed-4593317
recordtype oai_dc
spelling pubmed-45933172015-10-05 DNA Ligase I Is Not Essential for Mammalian Cell Viability Han, Li Masani, Shahnaz Hsieh, Chih-lin Yu, Kefei Article Of the three DNA ligases present in all vertebrates, DNA ligase I (Lig1) has been considered essential for ligating Okazaki fragments during DNA replication and thereby essential for cell viability. Here, we report the striking finding that a Lig1-null murine B cell line is viable. Surprisingly, the Lig1-null cells exhibit normal proliferation and normal immunoglobulin heavy chain class switch recombination and are not hypersensitive to a wide variety of DNA damaging agents. These findings demonstrate that Lig1 is not absolutely required for cellular DNA replication and repair and that either Lig3 or Lig4 can substitute for the role of Lig1 in joining Okazaki fragments. The establishment of a Lig1-null cell line will greatly facilitate the characterization of DNA ligase function in mammalian cells, but the finding alone profoundly reprioritizes the role of ligase I in DNA replication, repair, and recombination. 2014-04-13 2014-04-24 /pmc/articles/PMC4593317/ /pubmed/24726358 http://dx.doi.org/10.1016/j.celrep.2014.03.024 Text en http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Han, Li
Masani, Shahnaz
Hsieh, Chih-lin
Yu, Kefei
spellingShingle Han, Li
Masani, Shahnaz
Hsieh, Chih-lin
Yu, Kefei
DNA Ligase I Is Not Essential for Mammalian Cell Viability
author_facet Han, Li
Masani, Shahnaz
Hsieh, Chih-lin
Yu, Kefei
author_sort Han, Li
title DNA Ligase I Is Not Essential for Mammalian Cell Viability
title_short DNA Ligase I Is Not Essential for Mammalian Cell Viability
title_full DNA Ligase I Is Not Essential for Mammalian Cell Viability
title_fullStr DNA Ligase I Is Not Essential for Mammalian Cell Viability
title_full_unstemmed DNA Ligase I Is Not Essential for Mammalian Cell Viability
title_sort dna ligase i is not essential for mammalian cell viability
description Of the three DNA ligases present in all vertebrates, DNA ligase I (Lig1) has been considered essential for ligating Okazaki fragments during DNA replication and thereby essential for cell viability. Here, we report the striking finding that a Lig1-null murine B cell line is viable. Surprisingly, the Lig1-null cells exhibit normal proliferation and normal immunoglobulin heavy chain class switch recombination and are not hypersensitive to a wide variety of DNA damaging agents. These findings demonstrate that Lig1 is not absolutely required for cellular DNA replication and repair and that either Lig3 or Lig4 can substitute for the role of Lig1 in joining Okazaki fragments. The establishment of a Lig1-null cell line will greatly facilitate the characterization of DNA ligase function in mammalian cells, but the finding alone profoundly reprioritizes the role of ligase I in DNA replication, repair, and recombination.
publishDate 2014
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4593317/
_version_ 1613483488805126144

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