Development of a Tightly Controlled Off Switch for Saccharomyces cerevisiae Regulated by Camphor, a Low-Cost Natural Product

Here we describe the engineering of a distant homolog of the Tet repressor, CamR, isolated from Pseudomonas putida, that is regulated by camphor, a very inexpensive small molecule (at micromolar concentrations) for use in Saccharomyces cerevisiae. The repressor was engineered by expression from a co...

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Bibliographic Details
Main Authors: Ikushima, Shigehito, Zhao, Yu, Boeke, Jef D.
Format: Online
Language:English
Published: Genetics Society of America 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592980/