Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase

This paper was aimed to investigate the possible implications of the lack of plastidic glutamine synthetase (GS2) in phenolic metabolism during stress responses in the model legume Lotus japonicus. Important changes in the transcriptome were detected in a GS2 mutant called Ljgln2-2, compared to the...

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Main Authors:	García-Calderón, Margarita, Pons-Ferrer, Teresa, Mrázova, Anna, Pal'ove-Balang, Peter, Vilková, Mária, Pérez-Delgado, Carmen M., Vega, José M., Eliášová, Adriana, Repčák, Miroslav, Márquez, Antonio J., Betti, Marco
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585329/

id	pubmed-4585329
recordtype	oai_dc
spelling	pubmed-45853292015-10-05 Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase García-Calderón, Margarita Pons-Ferrer, Teresa Mrázova, Anna Pal'ove-Balang, Peter Vilková, Mária Pérez-Delgado, Carmen M. Vega, José M. Eliášová, Adriana Repčák, Miroslav Márquez, Antonio J. Betti, Marco Plant Science This paper was aimed to investigate the possible implications of the lack of plastidic glutamine synthetase (GS2) in phenolic metabolism during stress responses in the model legume Lotus japonicus. Important changes in the transcriptome were detected in a GS2 mutant called Ljgln2-2, compared to the wild type, in response to two separate stress conditions, such as drought or the result of the impairment of the photorespiratory cycle. Detailed transcriptomic analysis showed that the biosynthesis of phenolic compounds was affected in the mutant plants in these two different types of stress situations. For this reason, the genes and metabolites related to this metabolic route were further investigated using a combined approach of gene expression analysis and metabolite profiling. A high induction of the expression of several genes for the biosynthesis of different branches of the phenolic biosynthetic pathway was detected by qRT-PCR. The extent of induction was always higher in Ljgln2-2, probably reflecting the higher stress levels present in this genotype. This was paralleled by accumulation of several kaempferol and quercetine glycosides, some of them described for the first time in L. japonicus, and of high levels of the isoflavonoid vestitol. The results obtained indicate that the absence of GS2 affects different aspects of phenolic metabolism in L. japonicus plants in response to stress. Frontiers Media S.A. 2015-09-25 /pmc/articles/PMC4585329/ /pubmed/26442073 http://dx.doi.org/10.3389/fpls.2015.00760 Text en Copyright © 2015 García-Calderón, Pons-Ferrer, Mrázova, Pal'ove-Balang, Vilková, Pérez-Delgado, Vega, Eliášová, Repèák, Márquez and Betti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	García-Calderón, Margarita Pons-Ferrer, Teresa Mrázova, Anna Pal'ove-Balang, Peter Vilková, Mária Pérez-Delgado, Carmen M. Vega, José M. Eliášová, Adriana Repčák, Miroslav Márquez, Antonio J. Betti, Marco
spellingShingle	García-Calderón, Margarita Pons-Ferrer, Teresa Mrázova, Anna Pal'ove-Balang, Peter Vilková, Mária Pérez-Delgado, Carmen M. Vega, José M. Eliášová, Adriana Repčák, Miroslav Márquez, Antonio J. Betti, Marco Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase
author_facet	García-Calderón, Margarita Pons-Ferrer, Teresa Mrázova, Anna Pal'ove-Balang, Peter Vilková, Mária Pérez-Delgado, Carmen M. Vega, José M. Eliášová, Adriana Repčák, Miroslav Márquez, Antonio J. Betti, Marco
author_sort	García-Calderón, Margarita
title	Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase
title_short	Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase
title_full	Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase
title_fullStr	Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase
title_full_unstemmed	Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase
title_sort	modulation of phenolic metabolism under stress conditions in a lotus japonicus mutant lacking plastidic glutamine synthetase
description	This paper was aimed to investigate the possible implications of the lack of plastidic glutamine synthetase (GS2) in phenolic metabolism during stress responses in the model legume Lotus japonicus. Important changes in the transcriptome were detected in a GS2 mutant called Ljgln2-2, compared to the wild type, in response to two separate stress conditions, such as drought or the result of the impairment of the photorespiratory cycle. Detailed transcriptomic analysis showed that the biosynthesis of phenolic compounds was affected in the mutant plants in these two different types of stress situations. For this reason, the genes and metabolites related to this metabolic route were further investigated using a combined approach of gene expression analysis and metabolite profiling. A high induction of the expression of several genes for the biosynthesis of different branches of the phenolic biosynthetic pathway was detected by qRT-PCR. The extent of induction was always higher in Ljgln2-2, probably reflecting the higher stress levels present in this genotype. This was paralleled by accumulation of several kaempferol and quercetine glycosides, some of them described for the first time in L. japonicus, and of high levels of the isoflavonoid vestitol. The results obtained indicate that the absence of GS2 affects different aspects of phenolic metabolism in L. japonicus plants in response to stress.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585329/
_version_	1613480775164887040

Modulation of phenolic metabolism under stress conditions in a Lotus japonicus mutant lacking plastidic glutamine synthetase

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