Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells

The aim of the present study was to explore the preparation of arsenic trioxide (As2O3) nanoparticles and examine the antitumor effects of these nanoparticles on NB4 cells. As2O3 nanoparticles were prepared using the sol-gel method and characterized using transmission electron microscopy and energy...

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Main Authors:	DONG, XIAOYAN, MA, NING, LIU, MENGMENG, LIU, ZILING
Format:	Online
Language:	English
Published:	D.A. Spandidos 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578042/

id	pubmed-4578042
recordtype	oai_dc
spelling	pubmed-45780422015-11-30 Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells DONG, XIAOYAN MA, NING LIU, MENGMENG LIU, ZILING Articles The aim of the present study was to explore the preparation of arsenic trioxide (As2O3) nanoparticles and examine the antitumor effects of these nanoparticles on NB4 cells. As2O3 nanoparticles were prepared using the sol-gel method and characterized using transmission electron microscopy and energy dispersive spectroscopy. The results indicated that the As2O3 nanoparticles prepared in the present study were round or elliptical, well dispersed and had an ~40-nm or <10-nm diameter. The antitumor effects of As2O3 nanoparticles at various concentrations were analyzed by flow cytometry and the MTT assay, and were compared with those of traditional As2O3 solution. At the same concentration and incubation time (48 h), the survival rate of cells treated with As2O3 nanoparticles was significantly lower than that of cells treated with the As2O3 solution. The growth inhibition rate under both treatments was time- and dose-dependent. In addition, at the same concentration and incubation time, the apoptosis rate of the cells treated with As2O3 nanoparticles was significantly higher than that of the cells treated with the As2O3 solution. Furthermore, As2O3 nanoparticles resulted in a greater reduction in the expression of the anti-apoptotic protein B-cell lymphoma 2 compared with the As2O3 solution. In conclusion, As2O3 nanoparticles, prepared using the sol-gel method, were found to produce a stronger cytotoxic effect on tumor cells than that produced by the As2O3 solution, possibly by inhibiting Bcl-2 expression. D.A. Spandidos 2015-10 2015-07-23 /pmc/articles/PMC4578042/ /pubmed/26622477 http://dx.doi.org/10.3892/etm.2015.2651 Text en Copyright: © Dong et al. This is an open access article distributed under the terms of a Creative Commons Attribution License. http://creativecommons.org/licenses/by/4.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	DONG, XIAOYAN MA, NING LIU, MENGMENG LIU, ZILING
spellingShingle	DONG, XIAOYAN MA, NING LIU, MENGMENG LIU, ZILING Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells
author_facet	DONG, XIAOYAN MA, NING LIU, MENGMENG LIU, ZILING
author_sort	DONG, XIAOYAN
title	Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells
title_short	Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells
title_full	Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells
title_fullStr	Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells
title_full_unstemmed	Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells
title_sort	effects of as2o3 nanoparticles on cell growth and apoptosis of nb4 cells
description	The aim of the present study was to explore the preparation of arsenic trioxide (As2O3) nanoparticles and examine the antitumor effects of these nanoparticles on NB4 cells. As2O3 nanoparticles were prepared using the sol-gel method and characterized using transmission electron microscopy and energy dispersive spectroscopy. The results indicated that the As2O3 nanoparticles prepared in the present study were round or elliptical, well dispersed and had an ~40-nm or <10-nm diameter. The antitumor effects of As2O3 nanoparticles at various concentrations were analyzed by flow cytometry and the MTT assay, and were compared with those of traditional As2O3 solution. At the same concentration and incubation time (48 h), the survival rate of cells treated with As2O3 nanoparticles was significantly lower than that of cells treated with the As2O3 solution. The growth inhibition rate under both treatments was time- and dose-dependent. In addition, at the same concentration and incubation time, the apoptosis rate of the cells treated with As2O3 nanoparticles was significantly higher than that of the cells treated with the As2O3 solution. Furthermore, As2O3 nanoparticles resulted in a greater reduction in the expression of the anti-apoptotic protein B-cell lymphoma 2 compared with the As2O3 solution. In conclusion, As2O3 nanoparticles, prepared using the sol-gel method, were found to produce a stronger cytotoxic effect on tumor cells than that produced by the As2O3 solution, possibly by inhibiting Bcl-2 expression.
publisher	D.A. Spandidos
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578042/
_version_	1613478056784035840

Effects of As2O3 nanoparticles on cell growth and apoptosis of NB4 cells

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