Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis

In mice, hematopoietic cells home to bone marrow from fetal liver prenatally. To elucidate mechanisms underlying homing, we performed immunohistochemistry with the hematopoietic cell marker c-Kit, and observed c-Kit(+) cells localized inside muscle surrounding bone after 14.5 days post coitum. Flow...

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Main Authors:	Tanaka, Yuka, Inoue-Yokoo, Tomoko, Kulkeaw, Kasem, Yanagi-Mizuochi, Chiyo, Shirasawa, Senji, Nakanishi, Yoichi, Sugiyama, Daisuke
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577119/

id	pubmed-4577119
recordtype	oai_dc
spelling	pubmed-45771192015-09-25 Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis Tanaka, Yuka Inoue-Yokoo, Tomoko Kulkeaw, Kasem Yanagi-Mizuochi, Chiyo Shirasawa, Senji Nakanishi, Yoichi Sugiyama, Daisuke Research Article In mice, hematopoietic cells home to bone marrow from fetal liver prenatally. To elucidate mechanisms underlying homing, we performed immunohistochemistry with the hematopoietic cell marker c-Kit, and observed c-Kit(+) cells localized inside muscle surrounding bone after 14.5 days post coitum. Flow cytometric analysis showed that CD45(+) c-Kit(+) hematopoietic cells were more abundant in muscle than in bone marrow between 14.5 and 17.5 days post coitum, peaking at 16.5 days post coitum. CD45(+) c-Kit(+) cells in muscle at 16.5 days post coitum exhibited higher expression of Gata2, among several hematopoietic genes, than did fetal liver or bone marrow cells. Colony formation assays revealed that muscle hematopoietic cells possess hematopoietic progenitor activity. Furthermore, exo utero transplantation revealed that fetal liver hematopoietic progenitor cells home to muscle and then to BM. Our findings demonstrate that hematopoietic progenitor cell homing occurs earlier than previously reported and that hematopoietic progenitor cells reside in muscle tissue before bone marrow hematopoiesis occurs during mouse embryogenesis. Public Library of Science 2015-09-21 /pmc/articles/PMC4577119/ /pubmed/26389592 http://dx.doi.org/10.1371/journal.pone.0138621 Text en © 2015 Tanaka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tanaka, Yuka Inoue-Yokoo, Tomoko Kulkeaw, Kasem Yanagi-Mizuochi, Chiyo Shirasawa, Senji Nakanishi, Yoichi Sugiyama, Daisuke
spellingShingle	Tanaka, Yuka Inoue-Yokoo, Tomoko Kulkeaw, Kasem Yanagi-Mizuochi, Chiyo Shirasawa, Senji Nakanishi, Yoichi Sugiyama, Daisuke Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis
author_facet	Tanaka, Yuka Inoue-Yokoo, Tomoko Kulkeaw, Kasem Yanagi-Mizuochi, Chiyo Shirasawa, Senji Nakanishi, Yoichi Sugiyama, Daisuke
author_sort	Tanaka, Yuka
title	Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis
title_short	Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis
title_full	Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis
title_fullStr	Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis
title_full_unstemmed	Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis
title_sort	embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis
description	In mice, hematopoietic cells home to bone marrow from fetal liver prenatally. To elucidate mechanisms underlying homing, we performed immunohistochemistry with the hematopoietic cell marker c-Kit, and observed c-Kit(+) cells localized inside muscle surrounding bone after 14.5 days post coitum. Flow cytometric analysis showed that CD45(+) c-Kit(+) hematopoietic cells were more abundant in muscle than in bone marrow between 14.5 and 17.5 days post coitum, peaking at 16.5 days post coitum. CD45(+) c-Kit(+) cells in muscle at 16.5 days post coitum exhibited higher expression of Gata2, among several hematopoietic genes, than did fetal liver or bone marrow cells. Colony formation assays revealed that muscle hematopoietic cells possess hematopoietic progenitor activity. Furthermore, exo utero transplantation revealed that fetal liver hematopoietic progenitor cells home to muscle and then to BM. Our findings demonstrate that hematopoietic progenitor cell homing occurs earlier than previously reported and that hematopoietic progenitor cells reside in muscle tissue before bone marrow hematopoiesis occurs during mouse embryogenesis.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577119/
_version_	1613477804093997056

Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis

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