Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis

Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis

Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens. The present study aimed to investigate the association of the FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility to pulm...

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Main Authors: Xu, Dan-Dan, Wang, Chong, Jiang, Feng, Wei, Li-Liang, Shi, Li-Ying, Yu, Xiao-Mei, Liu, Chang-Ming, Liu, Xue-Hong, Feng, Xian-Min, Ping, Ze-Peng, Jiang, Ting-Ting, Chen, Zhong-Liang, Li, Zhong-Jie, Li, Ji-Cheng
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574923/
id pubmed-4574923
recordtype oai_dc
spelling pubmed-45749232015-09-25 Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis Xu, Dan-Dan Wang, Chong Jiang, Feng Wei, Li-Liang Shi, Li-Ying Yu, Xiao-Mei Liu, Chang-Ming Liu, Xue-Hong Feng, Xian-Min Ping, Ze-Peng Jiang, Ting-Ting Chen, Zhong-Liang Li, Zhong-Jie Li, Ji-Cheng Research Article Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens. The present study aimed to investigate the association of the FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility to pulmonary tuberculosis (TB). A total of seven SNPs in exon 8 (+6359 C>T and +6424 G>T) and in the promoter region (-986 G>A, -602 G>A, -557 A>G, -64 A>C and -4 A>G) of the FCN2 gene were genotyped using the PCR amplification and DNA sequencing methods in the healthy controls group (n = 254) and the pulmonary TB group (n = 282). The correlation between SNPs and pulmonary TB was analyzed using the logistic regression method. The results showed that there were no significant differences in the distribution of allelic frequencies of seven SNPs between the pulmonary TB group and the healthy controls group. However, the frequency of the variant homozygous genotype (P = 0.037, -557 A>G; P = 0.038, -64 A>C; P = 0.024, +6424 G>T) in the TB group was significantly lower than the control group. After adjustment for age and gender, these variant homozygous genotypes were found to be recessive models in association with pulmonary TB. In addition, -64 A>C (P = 0.047) and +6424 G>T (P = 0.03) were found to be codominant models in association with pulmonary TB. There was strong linkage disequilibrium (r2 > 0.80, P < 0.0001) between 7 SNPs except the -602 G>A site. Therefore, -557 A>G, -64 A>C and +6424 G>T SNPs of the FCN2 gene were correlated with pulmonary TB, and may be protective factors for TB. This study provides a novel idea for the prevention and control of TB transmission from a genetics perspective. Public Library of Science 2015-09-17 /pmc/articles/PMC4574923/ /pubmed/26379154 http://dx.doi.org/10.1371/journal.pone.0138356 Text en © 2015 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Xu, Dan-Dan
Wang, Chong
Jiang, Feng
Wei, Li-Liang
Shi, Li-Ying
Yu, Xiao-Mei
Liu, Chang-Ming
Liu, Xue-Hong
Feng, Xian-Min
Ping, Ze-Peng
Jiang, Ting-Ting
Chen, Zhong-Liang
Li, Zhong-Jie
Li, Ji-Cheng
spellingShingle Xu, Dan-Dan
Wang, Chong
Jiang, Feng
Wei, Li-Liang
Shi, Li-Ying
Yu, Xiao-Mei
Liu, Chang-Ming
Liu, Xue-Hong
Feng, Xian-Min
Ping, Ze-Peng
Jiang, Ting-Ting
Chen, Zhong-Liang
Li, Zhong-Jie
Li, Ji-Cheng
Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis
author_facet Xu, Dan-Dan
Wang, Chong
Jiang, Feng
Wei, Li-Liang
Shi, Li-Ying
Yu, Xiao-Mei
Liu, Chang-Ming
Liu, Xue-Hong
Feng, Xian-Min
Ping, Ze-Peng
Jiang, Ting-Ting
Chen, Zhong-Liang
Li, Zhong-Jie
Li, Ji-Cheng
author_sort Xu, Dan-Dan
title Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis
title_short Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis
title_full Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis
title_fullStr Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis
title_full_unstemmed Association of the FCN2 Gene Single Nucleotide Polymorphisms with Susceptibility to Pulmonary Tuberculosis
title_sort association of the fcn2 gene single nucleotide polymorphisms with susceptibility to pulmonary tuberculosis
description Ficolin-2 (FCN2) is an innate immune pattern recognition molecule that can activate the complement pathway, opsonophagocytosis, and elimination of the pathogens. The present study aimed to investigate the association of the FCN2 gene single nucleotide polymorphisms (SNPs) with susceptibility to pulmonary tuberculosis (TB). A total of seven SNPs in exon 8 (+6359 C>T and +6424 G>T) and in the promoter region (-986 G>A, -602 G>A, -557 A>G, -64 A>C and -4 A>G) of the FCN2 gene were genotyped using the PCR amplification and DNA sequencing methods in the healthy controls group (n = 254) and the pulmonary TB group (n = 282). The correlation between SNPs and pulmonary TB was analyzed using the logistic regression method. The results showed that there were no significant differences in the distribution of allelic frequencies of seven SNPs between the pulmonary TB group and the healthy controls group. However, the frequency of the variant homozygous genotype (P = 0.037, -557 A>G; P = 0.038, -64 A>C; P = 0.024, +6424 G>T) in the TB group was significantly lower than the control group. After adjustment for age and gender, these variant homozygous genotypes were found to be recessive models in association with pulmonary TB. In addition, -64 A>C (P = 0.047) and +6424 G>T (P = 0.03) were found to be codominant models in association with pulmonary TB. There was strong linkage disequilibrium (r2 > 0.80, P < 0.0001) between 7 SNPs except the -602 G>A site. Therefore, -557 A>G, -64 A>C and +6424 G>T SNPs of the FCN2 gene were correlated with pulmonary TB, and may be protective factors for TB. This study provides a novel idea for the prevention and control of TB transmission from a genetics perspective.
publisher Public Library of Science
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574923/
_version_ 1613477195599052800

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