Plasmid carriage can limit bacteria–phage coevolution

Coevolution with bacteriophages is a major selective force shaping bacterial populations and communities. A variety of both environmental and genetic factors has been shown to influence the mode and tempo of bacteria–phage coevolution. Here, we test the effects that carriage of a large conjugative p...

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Main Authors:	Harrison, Ellie, Truman, Julie, Wright, Rosanna, Spiers, Andrew J., Paterson, Steve, Brockhurst, Michael A.
Format:	Online
Language:	English
Published:	The Royal Society 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571675/

id	pubmed-4571675
recordtype	oai_dc
spelling	pubmed-45716752015-09-28 Plasmid carriage can limit bacteria–phage coevolution Harrison, Ellie Truman, Julie Wright, Rosanna Spiers, Andrew J. Paterson, Steve Brockhurst, Michael A. Evolutionary Biology Coevolution with bacteriophages is a major selective force shaping bacterial populations and communities. A variety of both environmental and genetic factors has been shown to influence the mode and tempo of bacteria–phage coevolution. Here, we test the effects that carriage of a large conjugative plasmid, pQBR103, had on antagonistic coevolution between the bacterium Pseudomonas fluorescens and its phage, SBW25ϕ2. Plasmid carriage limited bacteria–phage coevolution; bacteria evolved lower phage-resistance and phages evolved lower infectivity in plasmid-carrying compared with plasmid-free populations. These differences were not explained by effects of plasmid carriage on the costs of phage resistance mutations. Surprisingly, in the presence of phages, plasmid carriage resulted in the evolution of high frequencies of mucoid bacterial colonies. Mucoidy can provide weak partial resistance against SBW25ϕ2, which may have limited selection for qualitative resistance mutations in our experiments. Taken together, our results suggest that plasmids can have evolutionary consequences for bacteria that go beyond the direct phenotypic effects of their accessory gene cargo. The Royal Society 2015-08 /pmc/articles/PMC4571675/ /pubmed/26268992 http://dx.doi.org/10.1098/rsbl.2015.0361 Text en © 2015 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Harrison, Ellie Truman, Julie Wright, Rosanna Spiers, Andrew J. Paterson, Steve Brockhurst, Michael A.
spellingShingle	Harrison, Ellie Truman, Julie Wright, Rosanna Spiers, Andrew J. Paterson, Steve Brockhurst, Michael A. Plasmid carriage can limit bacteria–phage coevolution
author_facet	Harrison, Ellie Truman, Julie Wright, Rosanna Spiers, Andrew J. Paterson, Steve Brockhurst, Michael A.
author_sort	Harrison, Ellie
title	Plasmid carriage can limit bacteria–phage coevolution
title_short	Plasmid carriage can limit bacteria–phage coevolution
title_full	Plasmid carriage can limit bacteria–phage coevolution
title_fullStr	Plasmid carriage can limit bacteria–phage coevolution
title_full_unstemmed	Plasmid carriage can limit bacteria–phage coevolution
title_sort	plasmid carriage can limit bacteria–phage coevolution
description	Coevolution with bacteriophages is a major selective force shaping bacterial populations and communities. A variety of both environmental and genetic factors has been shown to influence the mode and tempo of bacteria–phage coevolution. Here, we test the effects that carriage of a large conjugative plasmid, pQBR103, had on antagonistic coevolution between the bacterium Pseudomonas fluorescens and its phage, SBW25ϕ2. Plasmid carriage limited bacteria–phage coevolution; bacteria evolved lower phage-resistance and phages evolved lower infectivity in plasmid-carrying compared with plasmid-free populations. These differences were not explained by effects of plasmid carriage on the costs of phage resistance mutations. Surprisingly, in the presence of phages, plasmid carriage resulted in the evolution of high frequencies of mucoid bacterial colonies. Mucoidy can provide weak partial resistance against SBW25ϕ2, which may have limited selection for qualitative resistance mutations in our experiments. Taken together, our results suggest that plasmids can have evolutionary consequences for bacteria that go beyond the direct phenotypic effects of their accessory gene cargo.
publisher	The Royal Society
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571675/
_version_	1613476403719700480

Plasmid carriage can limit bacteria–phage coevolution

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