SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles

We recently showed that Rab11 is involved not only in formation of recycling vesicles containing the transferrin (Tfn)–transferrin receptor (TfnR) complex at perinuclear recycling endosomes but also in tethering of recycling vesicles to the plasma membrane (PM) in concert with the exocyst tethering...

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Main Authors:	Kubo, Keiji, Kobayashi, Minako, Nozaki, Shohei, Yagi, Chikako, Hatsuzawa, Kiyotaka, Katoh, Yohei, Shin, Hye-Won, Takahashi, Senye, Nakayama, Kazuhisa
Format:	Online
Language:	English
Published:	The Company of Biologists 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571095/

id	pubmed-4571095
recordtype	oai_dc
spelling	pubmed-45710952015-09-17 SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles Kubo, Keiji Kobayashi, Minako Nozaki, Shohei Yagi, Chikako Hatsuzawa, Kiyotaka Katoh, Yohei Shin, Hye-Won Takahashi, Senye Nakayama, Kazuhisa Research Article We recently showed that Rab11 is involved not only in formation of recycling vesicles containing the transferrin (Tfn)–transferrin receptor (TfnR) complex at perinuclear recycling endosomes but also in tethering of recycling vesicles to the plasma membrane (PM) in concert with the exocyst tethering complex. We here aimed at identifying SNARE proteins responsible for fusion of Tfn–TfnR-containing recycling vesicles with the PM, downstream of the exocyst. We showed that exocyst subunits, Sec6 and Sec8, can interact with SNAP23 and SNAP25, both of which are PM-localizing Qbc-SNAREs, and that depletion of SNAP23 and/or SNAP25 in HeLa cells suppresses fusion of Tfn–TfnR-containing vesicles with the PM, leading to accumulation of the vesicles at the cell periphery. We also found that VAMP2, an R-SNARE, is colocalized with endocytosed Tfn on punctate endosomal structures, and that its depletion in HeLa cells suppresses recycling vesicle exocytosis. These observations indicate that fusion of recycling vesicles with the PM downstream of the exocyst is mediated by SNAP23/25 and VAMP2, and provide novel insight into non-neuronal roles of VAMP2 and SNAP25. The Company of Biologists 2015-06-19 /pmc/articles/PMC4571095/ /pubmed/26092867 http://dx.doi.org/10.1242/bio.012146 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kubo, Keiji Kobayashi, Minako Nozaki, Shohei Yagi, Chikako Hatsuzawa, Kiyotaka Katoh, Yohei Shin, Hye-Won Takahashi, Senye Nakayama, Kazuhisa
spellingShingle	Kubo, Keiji Kobayashi, Minako Nozaki, Shohei Yagi, Chikako Hatsuzawa, Kiyotaka Katoh, Yohei Shin, Hye-Won Takahashi, Senye Nakayama, Kazuhisa SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles
author_facet	Kubo, Keiji Kobayashi, Minako Nozaki, Shohei Yagi, Chikako Hatsuzawa, Kiyotaka Katoh, Yohei Shin, Hye-Won Takahashi, Senye Nakayama, Kazuhisa
author_sort	Kubo, Keiji
title	SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles
title_short	SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles
title_full	SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles
title_fullStr	SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles
title_full_unstemmed	SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles
title_sort	snap23/25 and vamp2 mediate exocytic event of transferrin receptor-containing recycling vesicles
description	We recently showed that Rab11 is involved not only in formation of recycling vesicles containing the transferrin (Tfn)–transferrin receptor (TfnR) complex at perinuclear recycling endosomes but also in tethering of recycling vesicles to the plasma membrane (PM) in concert with the exocyst tethering complex. We here aimed at identifying SNARE proteins responsible for fusion of Tfn–TfnR-containing recycling vesicles with the PM, downstream of the exocyst. We showed that exocyst subunits, Sec6 and Sec8, can interact with SNAP23 and SNAP25, both of which are PM-localizing Qbc-SNAREs, and that depletion of SNAP23 and/or SNAP25 in HeLa cells suppresses fusion of Tfn–TfnR-containing vesicles with the PM, leading to accumulation of the vesicles at the cell periphery. We also found that VAMP2, an R-SNARE, is colocalized with endocytosed Tfn on punctate endosomal structures, and that its depletion in HeLa cells suppresses recycling vesicle exocytosis. These observations indicate that fusion of recycling vesicles with the PM downstream of the exocyst is mediated by SNAP23/25 and VAMP2, and provide novel insight into non-neuronal roles of VAMP2 and SNAP25.
publisher	The Company of Biologists
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571095/
_version_	1613476254267211776

SNAP23/25 and VAMP2 mediate exocytic event of transferrin receptor-containing recycling vesicles

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