Perilipin 4 in human skeletal muscle: localization and effect of physical activity

Perilipins (PLINs) coat the surface of lipid droplets and are important for the regulation of lipid turnover. Knowledge about the physiological role of the individual PLINs in skeletal muscle is limited although lipid metabolism is very important for muscle contraction. To determine the effect of lo...

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Main Authors:	Pourteymour, Shirin, Lee, Sindre, Langleite, Torgrim M, Eckardt, Kristin, Hjorth, Marit, Bindesbøll, Christian, Dalen, Knut T, Birkeland, Kåre I, Drevon, Christian A, Holen, Torgeir, Norheim, Frode
Format:	Online
Language:	English
Published:	John Wiley & Sons, Ltd 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562567/

id	pubmed-4562567
recordtype	oai_dc
spelling	pubmed-45625672015-09-14 Perilipin 4 in human skeletal muscle: localization and effect of physical activity Pourteymour, Shirin Lee, Sindre Langleite, Torgrim M Eckardt, Kristin Hjorth, Marit Bindesbøll, Christian Dalen, Knut T Birkeland, Kåre I Drevon, Christian A Holen, Torgeir Norheim, Frode Original Research Perilipins (PLINs) coat the surface of lipid droplets and are important for the regulation of lipid turnover. Knowledge about the physiological role of the individual PLINs in skeletal muscle is limited although lipid metabolism is very important for muscle contraction. To determine the effect of long-term exercise on PLINs expression, 26 middle-aged, sedentary men underwent 12 weeks combined endurance and strength training intervention. Muscle biopsies from m. vastus lateralis and subcutaneous adipose tissue were taken before and after the intervention and total gene expression was measured with deep mRNA sequencing. PLIN4 mRNA exhibited the highest expression of all five PLINs in both tissues, and the expression was significantly reduced after long-term exercise in skeletal muscle. Moreover, PLIN4 mRNA expression levels in muscle correlated with the expression of genes involved in de novo phospholipid biosynthesis, with muscular content of phosphatidylethanolamine and phosphatidylcholine, and with the content of subsarcolemmal lipid droplets. The PLIN4 protein was mainly located at the periphery of skeletal muscle fibers, with higher levels in slow-twitch as compared to fast-twitch skeletal muscle fibers. In summary, we report reduced expression of PLIN4 after long-term physical activity, and preferential slow-twitch skeletal muscle fibers and plasma membrane-associated PLIN4 location. John Wiley & Sons, Ltd 2015-08-11 /pmc/articles/PMC4562567/ /pubmed/26265748 http://dx.doi.org/10.14814/phy2.12481 Text en © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Pourteymour, Shirin Lee, Sindre Langleite, Torgrim M Eckardt, Kristin Hjorth, Marit Bindesbøll, Christian Dalen, Knut T Birkeland, Kåre I Drevon, Christian A Holen, Torgeir Norheim, Frode
spellingShingle	Pourteymour, Shirin Lee, Sindre Langleite, Torgrim M Eckardt, Kristin Hjorth, Marit Bindesbøll, Christian Dalen, Knut T Birkeland, Kåre I Drevon, Christian A Holen, Torgeir Norheim, Frode Perilipin 4 in human skeletal muscle: localization and effect of physical activity
author_facet	Pourteymour, Shirin Lee, Sindre Langleite, Torgrim M Eckardt, Kristin Hjorth, Marit Bindesbøll, Christian Dalen, Knut T Birkeland, Kåre I Drevon, Christian A Holen, Torgeir Norheim, Frode
author_sort	Pourteymour, Shirin
title	Perilipin 4 in human skeletal muscle: localization and effect of physical activity
title_short	Perilipin 4 in human skeletal muscle: localization and effect of physical activity
title_full	Perilipin 4 in human skeletal muscle: localization and effect of physical activity
title_fullStr	Perilipin 4 in human skeletal muscle: localization and effect of physical activity
title_full_unstemmed	Perilipin 4 in human skeletal muscle: localization and effect of physical activity
title_sort	perilipin 4 in human skeletal muscle: localization and effect of physical activity
description	Perilipins (PLINs) coat the surface of lipid droplets and are important for the regulation of lipid turnover. Knowledge about the physiological role of the individual PLINs in skeletal muscle is limited although lipid metabolism is very important for muscle contraction. To determine the effect of long-term exercise on PLINs expression, 26 middle-aged, sedentary men underwent 12 weeks combined endurance and strength training intervention. Muscle biopsies from m. vastus lateralis and subcutaneous adipose tissue were taken before and after the intervention and total gene expression was measured with deep mRNA sequencing. PLIN4 mRNA exhibited the highest expression of all five PLINs in both tissues, and the expression was significantly reduced after long-term exercise in skeletal muscle. Moreover, PLIN4 mRNA expression levels in muscle correlated with the expression of genes involved in de novo phospholipid biosynthesis, with muscular content of phosphatidylethanolamine and phosphatidylcholine, and with the content of subsarcolemmal lipid droplets. The PLIN4 protein was mainly located at the periphery of skeletal muscle fibers, with higher levels in slow-twitch as compared to fast-twitch skeletal muscle fibers. In summary, we report reduced expression of PLIN4 after long-term physical activity, and preferential slow-twitch skeletal muscle fibers and plasma membrane-associated PLIN4 location.
publisher	John Wiley & Sons, Ltd
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562567/
_version_	1613473535160745984

Perilipin 4 in human skeletal muscle: localization and effect of physical activity

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