Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei

Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei

Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis...

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Main Authors: Suttisunhakul, Vichaya, Chantratita, Narisara, Wikraiphat, Chanthiwa, Wuthiekanun, Vanaporn, Douglas, Zakiya, Day, Nicholas P. J., Limmathurotsakul, Direk, Brett, Paul J., Burtnick, Mary N.
Format: Online
Language:English
Published: The American Society of Tropical Medicine and Hygiene 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559694/
id pubmed-4559694
recordtype oai_dc
spelling pubmed-45596942015-09-21 Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei Suttisunhakul, Vichaya Chantratita, Narisara Wikraiphat, Chanthiwa Wuthiekanun, Vanaporn Douglas, Zakiya Day, Nicholas P. J. Limmathurotsakul, Direk Brett, Paul J. Burtnick, Mary N. Articles Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified B. pseudomallei O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (N = 143) and healthy donors from either endemic (N = 199) or non-endemic areas (N = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (P < 0.001) and IHA (69.5%) (P = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (P = 0.053), but was significantly lower than the IHA (67.6%) (P = 0.002). In contrast, all tests with U.S. donor serum were highly specific (≥ 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas. The American Society of Tropical Medicine and Hygiene 2015-09-02 /pmc/articles/PMC4559694/ /pubmed/26123956 http://dx.doi.org/10.4269/ajtmh.15-0114 Text en ©The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Suttisunhakul, Vichaya
Chantratita, Narisara
Wikraiphat, Chanthiwa
Wuthiekanun, Vanaporn
Douglas, Zakiya
Day, Nicholas P. J.
Limmathurotsakul, Direk
Brett, Paul J.
Burtnick, Mary N.
spellingShingle Suttisunhakul, Vichaya
Chantratita, Narisara
Wikraiphat, Chanthiwa
Wuthiekanun, Vanaporn
Douglas, Zakiya
Day, Nicholas P. J.
Limmathurotsakul, Direk
Brett, Paul J.
Burtnick, Mary N.
Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei
author_facet Suttisunhakul, Vichaya
Chantratita, Narisara
Wikraiphat, Chanthiwa
Wuthiekanun, Vanaporn
Douglas, Zakiya
Day, Nicholas P. J.
Limmathurotsakul, Direk
Brett, Paul J.
Burtnick, Mary N.
author_sort Suttisunhakul, Vichaya
title Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei
title_short Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei
title_full Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei
title_fullStr Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei
title_full_unstemmed Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei
title_sort evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei
description Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Diagnosis of melioidosis currently relies on the isolation of B. pseudomallei from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified B. pseudomallei O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (N = 143) and healthy donors from either endemic (N = 199) or non-endemic areas (N = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (P < 0.001) and IHA (69.5%) (P = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (P = 0.053), but was significantly lower than the IHA (67.6%) (P = 0.002). In contrast, all tests with U.S. donor serum were highly specific (≥ 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas.
publisher The American Society of Tropical Medicine and Hygiene
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559694/
_version_ 1613472701264953344

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