Whole Organism Genome Editing: Targeted Large DNA Insertion via ObLiGaRe Nonhomologous End-Joining in Vivo Capture
Targeted gene insertion is a goal of genome editing and has been performed in cultured cells but only in a handful of whole organisms. The existing method to integrate foreign DNA using the homologous recombination pathway is inherently low efficiency, and many systems are refractory to this method....
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Genetics Society of America
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555220/ |