Genomic Comparison of Translocating and Non-Translocating Escherichia coli

Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to t...

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Main Authors:	Bachmann, Nathan L., Katouli, Mohammad, Polkinghorne, Adam
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552563/

id	pubmed-4552563
recordtype	oai_dc
spelling	pubmed-45525632015-09-10 Genomic Comparison of Translocating and Non-Translocating Escherichia coli Bachmann, Nathan L. Katouli, Mohammad Polkinghorne, Adam Research Article Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to the mesenteric lymph nodes and the blood stream in both humans and animal models. In this study the genomes of four TEC strains isolated from the mesenteric lymph nodes of a fatal case of hospitalised patient (HMLN-1), blood of pigs after experimental shock (PC-1) and after non-lethal haemorrhage in rats (KIC-1 and KIC-2) were sequenced in order to identify the genes associated with their adhesion and/or translocation. To facilitate the comparison, the genomes of a non-adhering, non-translocating E. coli (46–4) and adhering but non-translocating E. coli (73–89) were also sequenced and compared. Whole genome comparison revealed that three (HMLN-1, PC-1 and KIC-2) of the four TEC strains carried a genomic island that encodes a Type 6 Secretion System that may contribute to adhesion of the bacteria to gut epithelial cells. The human TEC strain HMLN-1 also carried the invasion ibeA gene, which was absent in the animal TEC strains and is likely to be associated with host-specific translocation. Phylogenetic analysis revealed that the four TEC strains were distributed amongst three distinct E. coli phylogroups, which was supported by the presence of phylogroup specific fimbriae gene clusters. The genomic comparison has identified potential genes that can be targeted with knock-out experiments to further characterise the mechanisms of E. coli translocation. Public Library of Science 2015-08-28 /pmc/articles/PMC4552563/ /pubmed/26317913 http://dx.doi.org/10.1371/journal.pone.0137131 Text en © 2015 Bachmann et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Bachmann, Nathan L. Katouli, Mohammad Polkinghorne, Adam
spellingShingle	Bachmann, Nathan L. Katouli, Mohammad Polkinghorne, Adam Genomic Comparison of Translocating and Non-Translocating Escherichia coli
author_facet	Bachmann, Nathan L. Katouli, Mohammad Polkinghorne, Adam
author_sort	Bachmann, Nathan L.
title	Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_short	Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_full	Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_fullStr	Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_full_unstemmed	Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_sort	genomic comparison of translocating and non-translocating escherichia coli
description	Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to the mesenteric lymph nodes and the blood stream in both humans and animal models. In this study the genomes of four TEC strains isolated from the mesenteric lymph nodes of a fatal case of hospitalised patient (HMLN-1), blood of pigs after experimental shock (PC-1) and after non-lethal haemorrhage in rats (KIC-1 and KIC-2) were sequenced in order to identify the genes associated with their adhesion and/or translocation. To facilitate the comparison, the genomes of a non-adhering, non-translocating E. coli (46–4) and adhering but non-translocating E. coli (73–89) were also sequenced and compared. Whole genome comparison revealed that three (HMLN-1, PC-1 and KIC-2) of the four TEC strains carried a genomic island that encodes a Type 6 Secretion System that may contribute to adhesion of the bacteria to gut epithelial cells. The human TEC strain HMLN-1 also carried the invasion ibeA gene, which was absent in the animal TEC strains and is likely to be associated with host-specific translocation. Phylogenetic analysis revealed that the four TEC strains were distributed amongst three distinct E. coli phylogroups, which was supported by the presence of phylogroup specific fimbriae gene clusters. The genomic comparison has identified potential genes that can be targeted with knock-out experiments to further characterise the mechanisms of E. coli translocation.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552563/
_version_	1613470098287230976

Genomic Comparison of Translocating and Non-Translocating Escherichia coli

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