The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei

Homeodomain transcription factors regulate development of embryos and cellular physiology in adult systems. Paired-type homeodomain genes constitute a subclass that has been particularly implicated in establishment of neuronal identity in the mammalian nervous system. We isolated fragments of eight...

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Main Authors:	Burbach, J. Peter H., Hellemons, Anita J. C. G. M., Grant, Philip, Pant, Harish C.
Format:	Online
Language:	English
Published:	The Company of Biologists 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542286/

id	pubmed-4542286
recordtype	oai_dc
spelling	pubmed-45422862015-09-16 The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei Burbach, J. Peter H. Hellemons, Anita J. C. G. M. Grant, Philip Pant, Harish C. Research Article Homeodomain transcription factors regulate development of embryos and cellular physiology in adult systems. Paired-type homeodomain genes constitute a subclass that has been particularly implicated in establishment of neuronal identity in the mammalian nervous system. We isolated fragments of eight homeodomain genes of this subclass expressed in the stellate ganglion of the North Atlantic long finned squid Loligo pealei (lp) [Note: Loligo pealei has been officially renamed Doryteuthis pealei. For reasons of uniformity and clarity Loligo pealei (lp) is used here]. Of the most abundant ones, we cloned a full length cDNA which encoded the squid ortholog of the paired-type homeodomain proteins Phox2a/b. The homology of lpPhox2 to invertebrate and mammalian Phox2 was limited to the homeodomain. In contrast to mouse Phox2b, lpPhox2 was unable to transactivate the dopamine beta-hydroxylase (DBH) promoter in a heterologous mammalian transfection system. In vivo, lpPhox2 was expressed in the developing stellate ganglion of stage 27 squid embryos and continued to be expressed in the adult stellate neurons where expression was confined to the giant fiber lobe containing the neurons that form the giant axons. The expression of lpPhox was similarly timed and distributed as the Fmrf gene. Furthermore, the Fmrf upstream region contained putative Phox2a/b binding sites. These results suggest a role of lpPhox2 in the developmental specification of neuronal identity and regulation of neurons of the squid giant axon. The Company of Biologists 2015-06-26 /pmc/articles/PMC4542286/ /pubmed/26116657 http://dx.doi.org/10.1242/bio.012476 Text en © 2015. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Burbach, J. Peter H. Hellemons, Anita J. C. G. M. Grant, Philip Pant, Harish C.
spellingShingle	Burbach, J. Peter H. Hellemons, Anita J. C. G. M. Grant, Philip Pant, Harish C. The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei
author_facet	Burbach, J. Peter H. Hellemons, Anita J. C. G. M. Grant, Philip Pant, Harish C.
author_sort	Burbach, J. Peter H.
title	The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei
title_short	The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei
title_full	The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei
title_fullStr	The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei
title_full_unstemmed	The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei
title_sort	homeodomain transcription factor phox2 in the stellate ganglion of the squid loligo pealei
description	Homeodomain transcription factors regulate development of embryos and cellular physiology in adult systems. Paired-type homeodomain genes constitute a subclass that has been particularly implicated in establishment of neuronal identity in the mammalian nervous system. We isolated fragments of eight homeodomain genes of this subclass expressed in the stellate ganglion of the North Atlantic long finned squid Loligo pealei (lp) [Note: Loligo pealei has been officially renamed Doryteuthis pealei. For reasons of uniformity and clarity Loligo pealei (lp) is used here]. Of the most abundant ones, we cloned a full length cDNA which encoded the squid ortholog of the paired-type homeodomain proteins Phox2a/b. The homology of lpPhox2 to invertebrate and mammalian Phox2 was limited to the homeodomain. In contrast to mouse Phox2b, lpPhox2 was unable to transactivate the dopamine beta-hydroxylase (DBH) promoter in a heterologous mammalian transfection system. In vivo, lpPhox2 was expressed in the developing stellate ganglion of stage 27 squid embryos and continued to be expressed in the adult stellate neurons where expression was confined to the giant fiber lobe containing the neurons that form the giant axons. The expression of lpPhox was similarly timed and distributed as the Fmrf gene. Furthermore, the Fmrf upstream region contained putative Phox2a/b binding sites. These results suggest a role of lpPhox2 in the developmental specification of neuronal identity and regulation of neurons of the squid giant axon.
publisher	The Company of Biologists
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542286/
_version_	1613261636020207616

The homeodomain transcription factor Phox2 in the stellate ganglion of the squid Loligo pealei

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