Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering
The state-of-the-art procedure for gene insertions into Trichoderma reesei is a cotransformation of two plasmids, one bearing the gene of interest and the other a marker gene. This procedure yields up to 80% transformation efficiency, but both the number of integrated copies and the loci of insertio...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
American Society for Microbiology
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542242/ |