Competitive binding of antagonistic peptides fine-tunes stomatal patterning

Competitive binding of antagonistic peptides fine-tunes stomatal patterning

During development, cells interpret complex, often conflicting signals to make optimal decisions. Plant stomata, the cellular interface between a plant and the atmosphere, develop according to positional cues including a family of secreted peptides, EPIDERMAL PATTERNING FACTORS (EPFs). How these sig...

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Main Authors: Lee, Jin Suk, Hnilova, Marketa, Maes, Michal, Lin, Ya-Chen Lisa, Putarjunan, Aarthi, Han, Soon-Ki, Avila, Julian, U.Torii, Keiko
Format: Online
Language:English
Published: 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532310/
id pubmed-4532310
recordtype oai_dc
spelling pubmed-45323102015-12-25 Competitive binding of antagonistic peptides fine-tunes stomatal patterning Lee, Jin Suk Hnilova, Marketa Maes, Michal Lin, Ya-Chen Lisa Putarjunan, Aarthi Han, Soon-Ki Avila, Julian U.Torii, Keiko Article During development, cells interpret complex, often conflicting signals to make optimal decisions. Plant stomata, the cellular interface between a plant and the atmosphere, develop according to positional cues including a family of secreted peptides, EPIDERMAL PATTERNING FACTORS (EPFs). How these signaling peptides orchestrate pattern formation at a molecular level remains unclear. Here we report that Stomagen/EPF-LIKE9 peptide, which promotes stomatal development, requires ERECTA (ER)-family receptor kinases and interferes with the inhibition of stomatal development by the EPF2-ER module. Both EPF2 and Stomagen directly bind to ER and its co-receptor TOO MANY MOUTHS. Stomagen peptide competitively replaced EPF2 binding to ER. Furthermore, application of EPF2, but not Stomagen, elicited rapid phosphorylation of downstream signaling components in vivo. Our findings demonstrate how a plant receptor agonist and antagonist define inhibitory and inductive cues to fine-tune tissue patterning on the plant epidermis. 2015-06-17 2015-06-25 /pmc/articles/PMC4532310/ /pubmed/26083750 http://dx.doi.org/10.1038/nature14561 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Lee, Jin Suk
Hnilova, Marketa
Maes, Michal
Lin, Ya-Chen Lisa
Putarjunan, Aarthi
Han, Soon-Ki
Avila, Julian
U.Torii, Keiko
spellingShingle Lee, Jin Suk
Hnilova, Marketa
Maes, Michal
Lin, Ya-Chen Lisa
Putarjunan, Aarthi
Han, Soon-Ki
Avila, Julian
U.Torii, Keiko
Competitive binding of antagonistic peptides fine-tunes stomatal patterning
author_facet Lee, Jin Suk
Hnilova, Marketa
Maes, Michal
Lin, Ya-Chen Lisa
Putarjunan, Aarthi
Han, Soon-Ki
Avila, Julian
U.Torii, Keiko
author_sort Lee, Jin Suk
title Competitive binding of antagonistic peptides fine-tunes stomatal patterning
title_short Competitive binding of antagonistic peptides fine-tunes stomatal patterning
title_full Competitive binding of antagonistic peptides fine-tunes stomatal patterning
title_fullStr Competitive binding of antagonistic peptides fine-tunes stomatal patterning
title_full_unstemmed Competitive binding of antagonistic peptides fine-tunes stomatal patterning
title_sort competitive binding of antagonistic peptides fine-tunes stomatal patterning
description During development, cells interpret complex, often conflicting signals to make optimal decisions. Plant stomata, the cellular interface between a plant and the atmosphere, develop according to positional cues including a family of secreted peptides, EPIDERMAL PATTERNING FACTORS (EPFs). How these signaling peptides orchestrate pattern formation at a molecular level remains unclear. Here we report that Stomagen/EPF-LIKE9 peptide, which promotes stomatal development, requires ERECTA (ER)-family receptor kinases and interferes with the inhibition of stomatal development by the EPF2-ER module. Both EPF2 and Stomagen directly bind to ER and its co-receptor TOO MANY MOUTHS. Stomagen peptide competitively replaced EPF2 binding to ER. Furthermore, application of EPF2, but not Stomagen, elicited rapid phosphorylation of downstream signaling components in vivo. Our findings demonstrate how a plant receptor agonist and antagonist define inhibitory and inductive cues to fine-tune tissue patterning on the plant epidermis.
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4532310/
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