CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair
Precise genome editing by the Cas9 nuclease depends on exogenously provided templates for homologous recombination. Here, we compare oligonucleotides with short homology and circular DNA molecules with extensive homology to genomic targets as templates for homology-based repair of CRISPR/Cas9 induce...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Genetics Society of America
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528321/ |