Determination of the Cytosolic NADPH/NADP Ratio in Saccharomyces cerevisiae using Shikimate Dehydrogenase as Sensor Reaction
Eukaryotic metabolism is organised in complex networks of enzyme catalysed reactions which are distributed over different organelles. To quantify the compartmentalised reactions, quantitative measurements of relevant physiological variables in different compartments are needed, especially of cofacto...
Main Authors: | , , , , , , , |
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Format: | Online |
Language: | English |
Published: |
Nature Publishing Group
2015
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4525286/ |