Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice

The CRISPR/Cas9 system is an efficient tool used for genome editing in a variety of organisms. Despite several recent reports of successful targeted mutagenesis using the CRISPR/Cas9 system in plants, in each case the target gene of interest, the Cas9 expression system and guide-RNA (gRNA) used, and...

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Bibliographic Details
Main Authors: Mikami, Masafumi, Toki, Seiichi, Endo, Masaki
Format: Online
Language:English
Published: Springer Netherlands 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523696/