Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery
Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. In order to achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. T...
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2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523223/ |
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pubmed-45232232016-01-01 Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery Yano, Shuya Hiroshima, Yukihiko Maawy, Ali Kishimoto, Hiroyuki Suetsugu, Atsushi Miwa, Shinji Toneri, Makoto Yamamoto, Mako Katz, Matthew H.G. Fleming, Jason B. Urata, Yasuo Tazawa, Hiroshi Kagawa, Shunsuke Bouvet, Michael Fujiwara, Toshiyoshi Hoffman, Robert M. Article Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. In order to achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. The telomerase-dependent green fluorescent protein (GFP) containing adenovirus OBP401 was used to label the cancer cells of the pancreatic cancer PDOX. The PDOX was previously grown in a red fluorescent protein (RFP) transgenic mouse that stably labeled the PDOX stroma cells bright red. The color-coded PDOX model enabled FGS to completely resect the pancreatic tumors including stroma. Dual-colored FGS significantly prevented local recurrence, which bright-light surgery (BLS) or single color could not. FGS, with color-coded cancer and stroma cells has important potential for improving the outcome of recalcitrant cancer. 2015-06-19 2015-07 /pmc/articles/PMC4523223/ /pubmed/26088297 http://dx.doi.org/10.1038/cgt.2015.26 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Yano, Shuya Hiroshima, Yukihiko Maawy, Ali Kishimoto, Hiroyuki Suetsugu, Atsushi Miwa, Shinji Toneri, Makoto Yamamoto, Mako Katz, Matthew H.G. Fleming, Jason B. Urata, Yasuo Tazawa, Hiroshi Kagawa, Shunsuke Bouvet, Michael Fujiwara, Toshiyoshi Hoffman, Robert M. |
| spellingShingle |
Yano, Shuya Hiroshima, Yukihiko Maawy, Ali Kishimoto, Hiroyuki Suetsugu, Atsushi Miwa, Shinji Toneri, Makoto Yamamoto, Mako Katz, Matthew H.G. Fleming, Jason B. Urata, Yasuo Tazawa, Hiroshi Kagawa, Shunsuke Bouvet, Michael Fujiwara, Toshiyoshi Hoffman, Robert M. Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery |
| author_facet |
Yano, Shuya Hiroshima, Yukihiko Maawy, Ali Kishimoto, Hiroyuki Suetsugu, Atsushi Miwa, Shinji Toneri, Makoto Yamamoto, Mako Katz, Matthew H.G. Fleming, Jason B. Urata, Yasuo Tazawa, Hiroshi Kagawa, Shunsuke Bouvet, Michael Fujiwara, Toshiyoshi Hoffman, Robert M. |
| author_sort |
Yano, Shuya |
| title |
Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery |
| title_short |
Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery |
| title_full |
Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery |
| title_fullStr |
Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery |
| title_full_unstemmed |
Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery |
| title_sort |
color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (pdox) model of pancreatic cancer enhances fluorescence-guided surgery |
| description |
Precise fluorescence-guided surgery (FGS) for pancreatic cancer has the potential to greatly improve the outcome in this recalcitrant disease. In order to achieve this goal, we have used genetic reporters to color code cancer and stroma cells in a patient-derived orthotopic xenograft (PDOX) model. The telomerase-dependent green fluorescent protein (GFP) containing adenovirus OBP401 was used to label the cancer cells of the pancreatic cancer PDOX. The PDOX was previously grown in a red fluorescent protein (RFP) transgenic mouse that stably labeled the PDOX stroma cells bright red. The color-coded PDOX model enabled FGS to completely resect the pancreatic tumors including stroma. Dual-colored FGS significantly prevented local recurrence, which bright-light surgery (BLS) or single color could not. FGS, with color-coded cancer and stroma cells has important potential for improving the outcome of recalcitrant cancer. |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4523223/ |
| _version_ |
1613255259564539904 |