Effect of high glucose concentrations on human erythrocytes in vitro

Exposure to high glucose concentrations in vitro is often employed as a model for understanding erythrocyte modifications in diabetes. However, effects of such experiments may be affected by glucose consumption during prolonged incubation and changes of cellular parameters conditioned by impaired en...

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Main Authors:	Viskupicova, Jana, Blaskovic, Dusan, Galiniak, Sabina, Soszyński, Mirosław, Bartosz, Grzegorz, Horakova, Lubica, Sadowska-Bartosz, Izabela
Format:	Online
Language:	English
Published:	Elsevier 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506982/

id	pubmed-4506982
recordtype	oai_dc
spelling	pubmed-45069822015-07-21 Effect of high glucose concentrations on human erythrocytes in vitro Viskupicova, Jana Blaskovic, Dusan Galiniak, Sabina Soszyński, Mirosław Bartosz, Grzegorz Horakova, Lubica Sadowska-Bartosz, Izabela Research Paper Exposure to high glucose concentrations in vitro is often employed as a model for understanding erythrocyte modifications in diabetes. However, effects of such experiments may be affected by glucose consumption during prolonged incubation and changes of cellular parameters conditioned by impaired energy balance. The aim of this study was to compare alterations in various red cell parameters in this type of experiment to differentiate between those affected by glycoxidation and those affected by energy imbalance. Erythrocytes were incubated with 5, 45 or 100 mM glucose for up to 72 h. High glucose concentrations intensified lipid peroxidation and loss of activities of erythrocyte enzymes (glutathione S-transferase and glutathione reductase). On the other hand, hemolysis, eryptosis, calcium accumulation, loss of glutathione and increase in the GSSG/GSH ratio were attenuated by high glucose apparently due to maintenance of energy supply to the cells. Loss of plasma membrane Ca2+-ATPase activity and decrease in superoxide production were not affected by glucose concentration, being seemingly determined by processes independent of both glycoxidation and energy depletion. These results point to the necessity of careful interpretation of data obtained in experiments, in which erythrocytes are subject to treatment with high glucose concentrations in vitro. Elsevier 2015-06-25 /pmc/articles/PMC4506982/ /pubmed/26141922 http://dx.doi.org/10.1016/j.redox.2015.06.011 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Viskupicova, Jana Blaskovic, Dusan Galiniak, Sabina Soszyński, Mirosław Bartosz, Grzegorz Horakova, Lubica Sadowska-Bartosz, Izabela
spellingShingle	Viskupicova, Jana Blaskovic, Dusan Galiniak, Sabina Soszyński, Mirosław Bartosz, Grzegorz Horakova, Lubica Sadowska-Bartosz, Izabela Effect of high glucose concentrations on human erythrocytes in vitro
author_facet	Viskupicova, Jana Blaskovic, Dusan Galiniak, Sabina Soszyński, Mirosław Bartosz, Grzegorz Horakova, Lubica Sadowska-Bartosz, Izabela
author_sort	Viskupicova, Jana
title	Effect of high glucose concentrations on human erythrocytes in vitro
title_short	Effect of high glucose concentrations on human erythrocytes in vitro
title_full	Effect of high glucose concentrations on human erythrocytes in vitro
title_fullStr	Effect of high glucose concentrations on human erythrocytes in vitro
title_full_unstemmed	Effect of high glucose concentrations on human erythrocytes in vitro
title_sort	effect of high glucose concentrations on human erythrocytes in vitro
description	Exposure to high glucose concentrations in vitro is often employed as a model for understanding erythrocyte modifications in diabetes. However, effects of such experiments may be affected by glucose consumption during prolonged incubation and changes of cellular parameters conditioned by impaired energy balance. The aim of this study was to compare alterations in various red cell parameters in this type of experiment to differentiate between those affected by glycoxidation and those affected by energy imbalance. Erythrocytes were incubated with 5, 45 or 100 mM glucose for up to 72 h. High glucose concentrations intensified lipid peroxidation and loss of activities of erythrocyte enzymes (glutathione S-transferase and glutathione reductase). On the other hand, hemolysis, eryptosis, calcium accumulation, loss of glutathione and increase in the GSSG/GSH ratio were attenuated by high glucose apparently due to maintenance of energy supply to the cells. Loss of plasma membrane Ca2+-ATPase activity and decrease in superoxide production were not affected by glucose concentration, being seemingly determined by processes independent of both glycoxidation and energy depletion. These results point to the necessity of careful interpretation of data obtained in experiments, in which erythrocytes are subject to treatment with high glucose concentrations in vitro.
publisher	Elsevier
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4506982/
_version_	1613249353724461056

Effect of high glucose concentrations on human erythrocytes in vitro

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