Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat

This study determined the effects of acute treatment with morphine on the expression of the Oprm1, Oprk1, and Oprd1 genes (which encode μ, κ, and δ receptors, respectively) in the striatum, hypothalamus, and periaqueductal gray (PAG) in ovariectomized female rats treated with estrogen. Ovariectomize...

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Main Authors:	Cruz, Wesley Soares, Pereira, Lucas Assis, Cezar, Luana Carvalho, Camarini, Rosana, Felicio, Luciano Freitas, Bernardi, Maria Martha, Teodorov, Elizabeth
Format:	Online
Language:	English
Published:	Springer International Publishing 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503706/

id	pubmed-4503706
recordtype	oai_dc
spelling	pubmed-45037062015-07-17 Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat Cruz, Wesley Soares Pereira, Lucas Assis Cezar, Luana Carvalho Camarini, Rosana Felicio, Luciano Freitas Bernardi, Maria Martha Teodorov, Elizabeth Research This study determined the effects of acute treatment with morphine on the expression of the Oprm1, Oprk1, and Oprd1 genes (which encode μ, κ, and δ receptors, respectively) in the striatum, hypothalamus, and periaqueductal gray (PAG) in ovariectomized female rats treated with estrogen. Ovariectomized female rats were divided into five equal groups. Two groups received estrogen (50 µg/kg, 54 h before testing) and saline (ES group) or 3.5 mg/kg morphine (EM group) 2 h before euthanasia. The SS group received saline solution 54 and 2 h before the experiments. The SM group received saline 54 h and 3.5 mg/kg morphine 2 h before the experiments. The W group remained undisturbed. The genes expression were evaluated. Oprm1 and Oprk1 expression were activated, respectively, in the hypothalamus and PAG and in the striatum and PAG by morphine only in estrogen-treated animals. Oprd1 expression in the hypothalamus and PAG was activated by morphine in both estrogen-treated and -nontreated animals. The Oprm1 and Oprk1 gene response to morphine might depend on estrogen, whereas the Oprd1 gene response to morphine might not depend on estrogen, supporting the hypothesis of a functional role for ovarian hormones in opioid receptor-mediated functional adaptations in the female brain. Springer International Publishing 2015-07-16 /pmc/articles/PMC4503706/ /pubmed/26191482 http://dx.doi.org/10.1186/s40064-015-1021-8 Text en © Cruz et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Cruz, Wesley Soares Pereira, Lucas Assis Cezar, Luana Carvalho Camarini, Rosana Felicio, Luciano Freitas Bernardi, Maria Martha Teodorov, Elizabeth
spellingShingle	Cruz, Wesley Soares Pereira, Lucas Assis Cezar, Luana Carvalho Camarini, Rosana Felicio, Luciano Freitas Bernardi, Maria Martha Teodorov, Elizabeth Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat
author_facet	Cruz, Wesley Soares Pereira, Lucas Assis Cezar, Luana Carvalho Camarini, Rosana Felicio, Luciano Freitas Bernardi, Maria Martha Teodorov, Elizabeth
author_sort	Cruz, Wesley Soares
title	Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat
title_short	Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat
title_full	Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat
title_fullStr	Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat
title_full_unstemmed	Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat
title_sort	role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat
description	This study determined the effects of acute treatment with morphine on the expression of the Oprm1, Oprk1, and Oprd1 genes (which encode μ, κ, and δ receptors, respectively) in the striatum, hypothalamus, and periaqueductal gray (PAG) in ovariectomized female rats treated with estrogen. Ovariectomized female rats were divided into five equal groups. Two groups received estrogen (50 µg/kg, 54 h before testing) and saline (ES group) or 3.5 mg/kg morphine (EM group) 2 h before euthanasia. The SS group received saline solution 54 and 2 h before the experiments. The SM group received saline 54 h and 3.5 mg/kg morphine 2 h before the experiments. The W group remained undisturbed. The genes expression were evaluated. Oprm1 and Oprk1 expression were activated, respectively, in the hypothalamus and PAG and in the striatum and PAG by morphine only in estrogen-treated animals. Oprd1 expression in the hypothalamus and PAG was activated by morphine in both estrogen-treated and -nontreated animals. The Oprm1 and Oprk1 gene response to morphine might depend on estrogen, whereas the Oprd1 gene response to morphine might not depend on estrogen, supporting the hypothesis of a functional role for ovarian hormones in opioid receptor-mediated functional adaptations in the female brain.
publisher	Springer International Publishing
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4503706/
_version_	1613248310555967488

Role of steroid hormones and morphine treatment in the modulation of opioid receptor gene expression in brain structures in the female rat

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