Blood group antigen studies using CdTe quantum dots and flow cytometry

New methods of analysis involving semiconductor nanocrystals (quantum dots [QDs]) as fluorescent probes have been highlighted in life science. QDs present some advantages when compared to organic dyes, such as size-tunable emission spectra, broad absorption bands, and principally exceptional resista...

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Main Authors:	Cabral Filho, Paulo E, Pereira, Maria IA, Fernandes, Heloise P, de Thomaz, Andre A, Cesar, Carlos L, Santos, Beate S, Barjas-Castro, Maria L, Fontes, Adriana
Format:	Online
Language:	English
Published:	Dove Medical Press 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501227/

id	pubmed-4501227
recordtype	oai_dc
spelling	pubmed-45012272015-07-16 Blood group antigen studies using CdTe quantum dots and flow cytometry Cabral Filho, Paulo E Pereira, Maria IA Fernandes, Heloise P de Thomaz, Andre A Cesar, Carlos L Santos, Beate S Barjas-Castro, Maria L Fontes, Adriana Original Research New methods of analysis involving semiconductor nanocrystals (quantum dots [QDs]) as fluorescent probes have been highlighted in life science. QDs present some advantages when compared to organic dyes, such as size-tunable emission spectra, broad absorption bands, and principally exceptional resistance to photobleaching. Methods applying QDs can be simple, not laborious, and can present high sensibility, allowing biomolecule identification and quantification with high specificity. In this context, the aim of this work was to apply dual-color CdTe QDs to quantify red blood cell (RBC) antigen expression on cell surface by flow cytometric analysis. QDs were conjugated to anti-A or anti-B monoclonal antibodies, as well as to the anti-H (Ulex europaeus I) lectin, to investigate RBCs of A1, B, A1B, O, A2, and Aweak donors. Bioconjugates were capable of distinguishing the different expressions of RBC antigens, both by labeling efficiency and by flow cytometry histogram profile. Furthermore, results showed that RBCs from Aweak donors present fewer amounts of A antigens and higher amounts of H, when compared to A1 RBCs. In the A group, the amount of A antigens decreased as A1 > A3 > AX = Ael, while H antigens were AX = Ael > A1. Bioconjugates presented stability and remained active for at least 6 months. In conclusion, this methodology with high sensibility and specificity can be applied to study a variety of RBC antigens, and, as a quantitative tool, can help in achieving a better comprehension of the antigen expression patterns on RBC membranes. Dove Medical Press 2015-07-08 /pmc/articles/PMC4501227/ /pubmed/26185442 http://dx.doi.org/10.2147/IJN.S84551 Text en © 2015 Cabral Filho et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Cabral Filho, Paulo E Pereira, Maria IA Fernandes, Heloise P de Thomaz, Andre A Cesar, Carlos L Santos, Beate S Barjas-Castro, Maria L Fontes, Adriana
spellingShingle	Cabral Filho, Paulo E Pereira, Maria IA Fernandes, Heloise P de Thomaz, Andre A Cesar, Carlos L Santos, Beate S Barjas-Castro, Maria L Fontes, Adriana Blood group antigen studies using CdTe quantum dots and flow cytometry
author_facet	Cabral Filho, Paulo E Pereira, Maria IA Fernandes, Heloise P de Thomaz, Andre A Cesar, Carlos L Santos, Beate S Barjas-Castro, Maria L Fontes, Adriana
author_sort	Cabral Filho, Paulo E
title	Blood group antigen studies using CdTe quantum dots and flow cytometry
title_short	Blood group antigen studies using CdTe quantum dots and flow cytometry
title_full	Blood group antigen studies using CdTe quantum dots and flow cytometry
title_fullStr	Blood group antigen studies using CdTe quantum dots and flow cytometry
title_full_unstemmed	Blood group antigen studies using CdTe quantum dots and flow cytometry
title_sort	blood group antigen studies using cdte quantum dots and flow cytometry
description	New methods of analysis involving semiconductor nanocrystals (quantum dots [QDs]) as fluorescent probes have been highlighted in life science. QDs present some advantages when compared to organic dyes, such as size-tunable emission spectra, broad absorption bands, and principally exceptional resistance to photobleaching. Methods applying QDs can be simple, not laborious, and can present high sensibility, allowing biomolecule identification and quantification with high specificity. In this context, the aim of this work was to apply dual-color CdTe QDs to quantify red blood cell (RBC) antigen expression on cell surface by flow cytometric analysis. QDs were conjugated to anti-A or anti-B monoclonal antibodies, as well as to the anti-H (Ulex europaeus I) lectin, to investigate RBCs of A1, B, A1B, O, A2, and Aweak donors. Bioconjugates were capable of distinguishing the different expressions of RBC antigens, both by labeling efficiency and by flow cytometry histogram profile. Furthermore, results showed that RBCs from Aweak donors present fewer amounts of A antigens and higher amounts of H, when compared to A1 RBCs. In the A group, the amount of A antigens decreased as A1 > A3 > AX = Ael, while H antigens were AX = Ael > A1. Bioconjugates presented stability and remained active for at least 6 months. In conclusion, this methodology with high sensibility and specificity can be applied to study a variety of RBC antigens, and, as a quantitative tool, can help in achieving a better comprehension of the antigen expression patterns on RBC membranes.
publisher	Dove Medical Press
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4501227/
_version_	1613247528080244736

Blood group antigen studies using CdTe quantum dots and flow cytometry

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