Placental amino acid transport may be regulated by maternal vitamin D and vitamin D-binding protein: results from the Southampton Women's Survey
Both maternal 25-hydroxyvitamin D (25(OH)D) concentrations during pregnancy and placental amino acid transporter gene expression have been associated with development of the offspring in terms of body composition and bone structure. Several amino acid transporter genes have vitamin D response elemen...
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| Format: | Online |
| Language: | English |
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Cambridge University Press
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498463/ |
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pubmed-44984632015-07-14 Placental amino acid transport may be regulated by maternal vitamin D and vitamin D-binding protein: results from the Southampton Women's Survey Cleal, J. K. Day, P. E. Simner, C. L. Barton, S. J. Mahon, P. A. Inskip, H. M. Godfrey, K. M. Hanson, M. A. Cooper, C. Lewis, R. M. Harvey, N. C. Full Papers Both maternal 25-hydroxyvitamin D (25(OH)D) concentrations during pregnancy and placental amino acid transporter gene expression have been associated with development of the offspring in terms of body composition and bone structure. Several amino acid transporter genes have vitamin D response elements in their promoters suggesting the possible linkage of these two mechanisms. We aimed to establish whether maternal 25(OH)D and vitamin D-binding protein (VDBP) levels relate to expression of placental amino acid transporters. RNA was extracted from 102 placental samples collected in the Southampton Women's Survey, and gene expression was analysed using quantitative real-time PCR. Gene expression data were normalised to the geometric mean of three housekeeping genes, and related to maternal factors and childhood body composition. Maternal serum 25(OH)D and VDBP levels were measured by radioimmunoassay. Maternal 25(OH)D and VDBP levels were positively associated with placental expression of specific genes involved in amino acid transport. Maternal 25(OH)D and VDBP concentrations were correlated with the expression of specific placental amino acid transporters, and thus may be involved in the regulation of amino acid transfer to the fetus. The positive correlation of VDBP levels and placental transporter expression suggests that delivery of vitamin D to the placenta may be important. This exploratory study identifies placental amino acid transporters which may be altered in response to modifiable maternal factors and provides a basis for further studies. Cambridge University Press 2015-06-28 2015-05-05 /pmc/articles/PMC4498463/ /pubmed/25940599 http://dx.doi.org/10.1017/S0007114515001178 Text en © The Authors 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Cleal, J. K. Day, P. E. Simner, C. L. Barton, S. J. Mahon, P. A. Inskip, H. M. Godfrey, K. M. Hanson, M. A. Cooper, C. Lewis, R. M. Harvey, N. C. |
| spellingShingle |
Cleal, J. K. Day, P. E. Simner, C. L. Barton, S. J. Mahon, P. A. Inskip, H. M. Godfrey, K. M. Hanson, M. A. Cooper, C. Lewis, R. M. Harvey, N. C. Placental amino acid transport may be regulated by maternal vitamin D and vitamin D-binding protein: results from the Southampton Women's Survey |
| author_facet |
Cleal, J. K. Day, P. E. Simner, C. L. Barton, S. J. Mahon, P. A. Inskip, H. M. Godfrey, K. M. Hanson, M. A. Cooper, C. Lewis, R. M. Harvey, N. C. |
| author_sort |
Cleal, J. K. |
| title |
Placental amino acid transport may be regulated by maternal vitamin D
and vitamin D-binding protein: results from the Southampton Women's
Survey |
| title_short |
Placental amino acid transport may be regulated by maternal vitamin D
and vitamin D-binding protein: results from the Southampton Women's
Survey |
| title_full |
Placental amino acid transport may be regulated by maternal vitamin D
and vitamin D-binding protein: results from the Southampton Women's
Survey |
| title_fullStr |
Placental amino acid transport may be regulated by maternal vitamin D
and vitamin D-binding protein: results from the Southampton Women's
Survey |
| title_full_unstemmed |
Placental amino acid transport may be regulated by maternal vitamin D
and vitamin D-binding protein: results from the Southampton Women's
Survey |
| title_sort |
placental amino acid transport may be regulated by maternal vitamin d
and vitamin d-binding protein: results from the southampton women's
survey |
| description |
Both maternal 25-hydroxyvitamin D (25(OH)D) concentrations during pregnancy and
placental amino acid transporter gene expression have been associated with
development of the offspring in terms of body composition and bone structure.
Several amino acid transporter genes have vitamin D response elements in their
promoters suggesting the possible linkage of these two mechanisms. We aimed to
establish whether maternal 25(OH)D and vitamin D-binding protein (VDBP) levels
relate to expression of placental amino acid transporters. RNA was extracted
from 102 placental samples collected in the Southampton Women's Survey,
and gene expression was analysed using quantitative real-time PCR. Gene
expression data were normalised to the geometric mean of three housekeeping
genes, and related to maternal factors and childhood body composition. Maternal
serum 25(OH)D and VDBP levels were measured by radioimmunoassay. Maternal
25(OH)D and VDBP levels were positively associated with placental expression of
specific genes involved in amino acid transport. Maternal 25(OH)D and VDBP
concentrations were correlated with the expression of specific placental amino
acid transporters, and thus may be involved in the regulation of amino acid
transfer to the fetus. The positive correlation of VDBP levels and placental
transporter expression suggests that delivery of vitamin D to the placenta may
be important. This exploratory study identifies placental amino acid
transporters which may be altered in response to modifiable maternal factors and
provides a basis for further studies. |
| publisher |
Cambridge University Press |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498463/ |
| _version_ |
1613246464886046720 |