Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development

Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplant...

Full description

Bibliographic Details
Main Authors:	OOGA, Masatoshi, SUZUKI, Masataka G., AOKI, Fugaku
Format:	Online
Language:	English
Published:	The Society for Reproduction and Development 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498372/

id	pubmed-4498372
recordtype	oai_dc
spelling	pubmed-44983722015-07-13 Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development OOGA, Masatoshi SUZUKI, Masataka G. AOKI, Fugaku Original Article Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplantation development. Based on immunocytochemical analysis using an anti-H2Bub1 antibody, no H2Bub1 signal was detected in the metaphase chromosomes of unfertilized oocytes or the pronuclei of early 1-cell stage embryos, but a weak signal was observed in late 1-cell stage embryos. The signal increased after cleavage into the 2-cell stage, and thereafter a strong signal was observed until the blastocyst stage. To assess the significance of H2Bub1 in the regulation of preimplantation development, RNF20 (an H2B-specific ubiquitin E3 ligase) was knocked down using small interfering RNA (siRNAs). In embryos treated with siRNA, the levels of Rnf20 mRNA and H2Bub1 decreased at the 4-cell and morula stages. Although these embryos developed normally until the morula stage, only one-third developed into the blastocyst stage. These results suggested that H2Bub1 is involved in the regulation of preimplantation development. The Society for Reproduction and Development 2015-02-06 2015-06 /pmc/articles/PMC4498372/ /pubmed/25740253 http://dx.doi.org/10.1262/jrd.2014-137 Text en ©2015 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	OOGA, Masatoshi SUZUKI, Masataka G. AOKI, Fugaku
spellingShingle	OOGA, Masatoshi SUZUKI, Masataka G. AOKI, Fugaku Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
author_facet	OOGA, Masatoshi SUZUKI, Masataka G. AOKI, Fugaku
author_sort	OOGA, Masatoshi
title	Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_short	Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_full	Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_fullStr	Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_full_unstemmed	Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development
title_sort	involvement of histone h2b monoubiquitination in the regulation of mouse preimplantation development
description	Histone H2B monoubiquitination (H2Bub1) plays an important role in developmental regulation in various vertebrate species. However, the role of H2Bub1 in mammalian preimplantation development remains unclear. In the present study, we examined the role of H2Bub1 in the regulation of mouse preimplantation development. Based on immunocytochemical analysis using an anti-H2Bub1 antibody, no H2Bub1 signal was detected in the metaphase chromosomes of unfertilized oocytes or the pronuclei of early 1-cell stage embryos, but a weak signal was observed in late 1-cell stage embryos. The signal increased after cleavage into the 2-cell stage, and thereafter a strong signal was observed until the blastocyst stage. To assess the significance of H2Bub1 in the regulation of preimplantation development, RNF20 (an H2B-specific ubiquitin E3 ligase) was knocked down using small interfering RNA (siRNAs). In embryos treated with siRNA, the levels of Rnf20 mRNA and H2Bub1 decreased at the 4-cell and morula stages. Although these embryos developed normally until the morula stage, only one-third developed into the blastocyst stage. These results suggested that H2Bub1 is involved in the regulation of preimplantation development.
publisher	The Society for Reproduction and Development
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498372/
_version_	1613246439817740288

Involvement of histone H2B monoubiquitination in the regulation of mouse preimplantation development

Similar Items