Valproic acid promotes radiosensitization in meningioma stem-like cells

Although meningioma stem-like cells have been isolated and characterized, their therapeutic targeting remains a challenge. Meningioma sphere cells (MgSCs) with cancer stem cells properties show chemo- and radioresistance in comparison with meningioma adherent cells (MgACs). We tested the effect of v...

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Main Authors:	Chiou, Hsin-Ying Clair, Lai, Wen-Kuo, Huang, Li-Chun, Huang, Shih-Ming, Chueh, Sheau-Huei, Ma, Hsin-I, Hueng, Dueng-Yuan
Format:	Online
Language:	English
Published:	Impact Journals LLC 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496410/

id	pubmed-4496410
recordtype	oai_dc
spelling	pubmed-44964102015-07-15 Valproic acid promotes radiosensitization in meningioma stem-like cells Chiou, Hsin-Ying Clair Lai, Wen-Kuo Huang, Li-Chun Huang, Shih-Ming Chueh, Sheau-Huei Ma, Hsin-I Hueng, Dueng-Yuan Research Paper Although meningioma stem-like cells have been isolated and characterized, their therapeutic targeting remains a challenge. Meningioma sphere cells (MgSCs) with cancer stem cells properties show chemo- and radioresistance in comparison with meningioma adherent cells (MgACs). We tested the effect of valproic acid (VPA), a commonly used anti-epileptic drug, which passes the blood brain barrier, on cultured MgSCs. VPA reduced the viability of MgSCs and MgACs. In MgSCs, treatment with VPA increased radio-sensitivity, expression of p-cdc2, p-H2AX and cleaved caspase-3 and PARP. Anchorage-independent growth (AIG) was reduced by VPA. AIG was further reduced by combined treatment with irradiation. Expression of a stem cell marker, Oct4, was reduced by VPA. Oct4 was further decreased by combined treatment with irradiation. These results suggest that VPA may be a potential treatment for meningioma through targeting meningioma stem-like cells. Impact Journals LLC 2015-03-29 /pmc/articles/PMC4496410/ /pubmed/25895030 Text en Copyright: © 2015 Chiou et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chiou, Hsin-Ying Clair Lai, Wen-Kuo Huang, Li-Chun Huang, Shih-Ming Chueh, Sheau-Huei Ma, Hsin-I Hueng, Dueng-Yuan
spellingShingle	Chiou, Hsin-Ying Clair Lai, Wen-Kuo Huang, Li-Chun Huang, Shih-Ming Chueh, Sheau-Huei Ma, Hsin-I Hueng, Dueng-Yuan Valproic acid promotes radiosensitization in meningioma stem-like cells
author_facet	Chiou, Hsin-Ying Clair Lai, Wen-Kuo Huang, Li-Chun Huang, Shih-Ming Chueh, Sheau-Huei Ma, Hsin-I Hueng, Dueng-Yuan
author_sort	Chiou, Hsin-Ying Clair
title	Valproic acid promotes radiosensitization in meningioma stem-like cells
title_short	Valproic acid promotes radiosensitization in meningioma stem-like cells
title_full	Valproic acid promotes radiosensitization in meningioma stem-like cells
title_fullStr	Valproic acid promotes radiosensitization in meningioma stem-like cells
title_full_unstemmed	Valproic acid promotes radiosensitization in meningioma stem-like cells
title_sort	valproic acid promotes radiosensitization in meningioma stem-like cells
description	Although meningioma stem-like cells have been isolated and characterized, their therapeutic targeting remains a challenge. Meningioma sphere cells (MgSCs) with cancer stem cells properties show chemo- and radioresistance in comparison with meningioma adherent cells (MgACs). We tested the effect of valproic acid (VPA), a commonly used anti-epileptic drug, which passes the blood brain barrier, on cultured MgSCs. VPA reduced the viability of MgSCs and MgACs. In MgSCs, treatment with VPA increased radio-sensitivity, expression of p-cdc2, p-H2AX and cleaved caspase-3 and PARP. Anchorage-independent growth (AIG) was reduced by VPA. AIG was further reduced by combined treatment with irradiation. Expression of a stem cell marker, Oct4, was reduced by VPA. Oct4 was further decreased by combined treatment with irradiation. These results suggest that VPA may be a potential treatment for meningioma through targeting meningioma stem-like cells.
publisher	Impact Journals LLC
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496410/
_version_	1613245876907540480

Valproic acid promotes radiosensitization in meningioma stem-like cells

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