Molecular phenotyping of plant single cell-types enhances forward genetic analyses
Recent advances in the isolation of single cell-types in plants provides an opportunity to conduct detailed analyses of their molecular characteristics at high resolution. This kind of cell-type specific molecular phenotyping is likely to enhance forward genetics studies to dissect the effect of mut...
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| Format: | Online |
| Language: | English |
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Frontiers Media S.A.
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491599/ |
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pubmed-44915992015-07-27 Molecular phenotyping of plant single cell-types enhances forward genetic analyses Schiefelbein, John Plant Science Recent advances in the isolation of single cell-types in plants provides an opportunity to conduct detailed analyses of their molecular characteristics at high resolution. This kind of cell-type specific molecular phenotyping is likely to enhance forward genetics studies to dissect the effect of mutations and thereby aid gene function assignment. Recent experimental results support this view, demonstrating that different cell-types exhibit substantial variation in transcript, protein, and metabolite accumulation and these molecular phenotypes are often sensitive to genetic and environmental alterations. The use of single cell-type molecular phenotyping approach to define plant gene function is most amenable to cell-types with well-characterized molecular tools and isolation protocols. Frontiers Media S.A. 2015-07-06 /pmc/articles/PMC4491599/ /pubmed/26217361 http://dx.doi.org/10.3389/fpls.2015.00509 Text en Copyright © 2015 Schiefelbein. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Schiefelbein, John |
| spellingShingle |
Schiefelbein, John Molecular phenotyping of plant single cell-types enhances forward genetic analyses |
| author_facet |
Schiefelbein, John |
| author_sort |
Schiefelbein, John |
| title |
Molecular phenotyping of plant single cell-types enhances forward genetic analyses |
| title_short |
Molecular phenotyping of plant single cell-types enhances forward genetic analyses |
| title_full |
Molecular phenotyping of plant single cell-types enhances forward genetic analyses |
| title_fullStr |
Molecular phenotyping of plant single cell-types enhances forward genetic analyses |
| title_full_unstemmed |
Molecular phenotyping of plant single cell-types enhances forward genetic analyses |
| title_sort |
molecular phenotyping of plant single cell-types enhances forward genetic analyses |
| description |
Recent advances in the isolation of single cell-types in plants provides an opportunity to conduct detailed analyses of their molecular characteristics at high resolution. This kind of cell-type specific molecular phenotyping is likely to enhance forward genetics studies to dissect the effect of mutations and thereby aid gene function assignment. Recent experimental results support this view, demonstrating that different cell-types exhibit substantial variation in transcript, protein, and metabolite accumulation and these molecular phenotypes are often sensitive to genetic and environmental alterations. The use of single cell-type molecular phenotyping approach to define plant gene function is most amenable to cell-types with well-characterized molecular tools and isolation protocols. |
| publisher |
Frontiers Media S.A. |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491599/ |
| _version_ |
1613243996126052352 |