Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid

We focus on the identification of complete and recombined ribosomal DNA-bearing chromosomes, and the dynamics of chromosomal number and position of ribosomal DNA (rDNA) loci in the F2-F4 generations derived from the F1 hybrid of Festuca pratensis Huds. (2n = 4x = 28) × Lolium perenne L. (2n = 4x = 2...

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Main Authors:	Książczyk, Tomasz, Zwierzykowska, Elżbieta, Molik, Katarzyna, Taciak, Magdalena, Krajewski, Paweł, Zwierzykowski, Zbigniew
Format:	Online
Language:	English
Published:	Springer Vienna 2014
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491343/

id	pubmed-4491343
recordtype	oai_dc
spelling	pubmed-44913432015-07-08 Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid Książczyk, Tomasz Zwierzykowska, Elżbieta Molik, Katarzyna Taciak, Magdalena Krajewski, Paweł Zwierzykowski, Zbigniew Original Article We focus on the identification of complete and recombined ribosomal DNA-bearing chromosomes, and the dynamics of chromosomal number and position of ribosomal DNA (rDNA) loci in the F2-F4 generations derived from the F1 hybrid of Festuca pratensis Huds. (2n = 4x = 28) × Lolium perenne L. (2n = 4x = 28). Lolium genomic DNA and rRNA genes were mapped by means of genomic and fluorescence in situ hybridization (GISH and FISH). The results revealed that plants of the three generations share various rDNA loci profiles with chromosome structural changes, possibly as a result of chromosomal inter- and intra-rearrangements. We observed an asymmetrical variation in the number of recombinant arms with and without rDNA loci between parental genomes. The Lolium genome was more affected by rearrangements in arms with rDNA loci, while Festuca was more affected in arms without them. Statistically significant differences between L. perenne and F. pratensis genomes concerned the number of recombined chromosomes without rDNA, and the number of recombined rDNA-bearing chromosomal arms of marked chromosomes, showing a tendency of F. pratensis genome-like chromosomes to be less stable, compared with L. perenne. We postulate a novel genome-dependent range and type of chromosome variation in plants of the F2-F4 generations derived from F. pratensis × L. perenne hybrid. Springer Vienna 2014-12-06 2015 /pmc/articles/PMC4491343/ /pubmed/25480732 http://dx.doi.org/10.1007/s00709-014-0734-9 Text en © The Author(s) 2014 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Książczyk, Tomasz Zwierzykowska, Elżbieta Molik, Katarzyna Taciak, Magdalena Krajewski, Paweł Zwierzykowski, Zbigniew
spellingShingle	Książczyk, Tomasz Zwierzykowska, Elżbieta Molik, Katarzyna Taciak, Magdalena Krajewski, Paweł Zwierzykowski, Zbigniew Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid
author_facet	Książczyk, Tomasz Zwierzykowska, Elżbieta Molik, Katarzyna Taciak, Magdalena Krajewski, Paweł Zwierzykowski, Zbigniew
author_sort	Książczyk, Tomasz
title	Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid
title_short	Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid
title_full	Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid
title_fullStr	Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid
title_full_unstemmed	Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid
title_sort	genome-dependent chromosome dynamics in three successive generations of the allotetraploid festuca pratensis × lolium perenne hybrid
description	We focus on the identification of complete and recombined ribosomal DNA-bearing chromosomes, and the dynamics of chromosomal number and position of ribosomal DNA (rDNA) loci in the F2-F4 generations derived from the F1 hybrid of Festuca pratensis Huds. (2n = 4x = 28) × Lolium perenne L. (2n = 4x = 28). Lolium genomic DNA and rRNA genes were mapped by means of genomic and fluorescence in situ hybridization (GISH and FISH). The results revealed that plants of the three generations share various rDNA loci profiles with chromosome structural changes, possibly as a result of chromosomal inter- and intra-rearrangements. We observed an asymmetrical variation in the number of recombinant arms with and without rDNA loci between parental genomes. The Lolium genome was more affected by rearrangements in arms with rDNA loci, while Festuca was more affected in arms without them. Statistically significant differences between L. perenne and F. pratensis genomes concerned the number of recombined chromosomes without rDNA, and the number of recombined rDNA-bearing chromosomal arms of marked chromosomes, showing a tendency of F. pratensis genome-like chromosomes to be less stable, compared with L. perenne. We postulate a novel genome-dependent range and type of chromosome variation in plants of the F2-F4 generations derived from F. pratensis × L. perenne hybrid.
publisher	Springer Vienna
publishDate	2014
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491343/
_version_	1613243885911277568

Genome-dependent chromosome dynamics in three successive generations of the allotetraploid Festuca pratensis × Lolium perenne hybrid

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