Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization

Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mechanisms th...

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Main Authors:	Winstel, Volker, Kühner, Petra, Salomon, Ferdinand, Larsen, Jesper, Skov, Robert, Hoffmann, Wolfgang, Peschel, Andreas, Weidenmaier, Christopher
Format:	Online
Language:	English
Published:	American Society of Microbiology 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488942/

id	pubmed-4488942
recordtype	oai_dc
spelling	pubmed-44889422015-07-07 Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization Winstel, Volker Kühner, Petra Salomon, Ferdinand Larsen, Jesper Skov, Robert Hoffmann, Wolfgang Peschel, Andreas Weidenmaier, Christopher Research Article Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mechanisms that govern WTA-mediated nasal colonization have remained elusive. Here, we report that WTA GlcNAcylation is a pivotal requirement for WTA-dependent attachment of community-acquired methicillin-resistant S. aureus (MRSA) and emerging livestock-associated MRSA to human nasal epithelial cells, even under conditions simulating the nutrient composition and dynamic flow of nasal secretions. Depending on the S. aureus strain, WTA O-GlcNAcylation occurs in either α or β configuration, which have similar capacities to mediate attachment to human nasal epithelial cells, suggesting that many S. aureus strains maintain redundant pathways to ensure appropriate WTA glycosylation. Strikingly, a lack of WTA glycosylation significantly abrogated the ability of MRSA to colonize cotton rat nares in vivo. These results indicate that WTA glycosylation modulates S. aureus nasal colonization and may help to develop new strategies for eradicating S. aureus nasal colonization in the future. American Society of Microbiology 2015-06-30 /pmc/articles/PMC4488942/ /pubmed/26126851 http://dx.doi.org/10.1128/mBio.00632-15 Text en Copyright © 2015 Winstel et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Winstel, Volker Kühner, Petra Salomon, Ferdinand Larsen, Jesper Skov, Robert Hoffmann, Wolfgang Peschel, Andreas Weidenmaier, Christopher
spellingShingle	Winstel, Volker Kühner, Petra Salomon, Ferdinand Larsen, Jesper Skov, Robert Hoffmann, Wolfgang Peschel, Andreas Weidenmaier, Christopher Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization
author_facet	Winstel, Volker Kühner, Petra Salomon, Ferdinand Larsen, Jesper Skov, Robert Hoffmann, Wolfgang Peschel, Andreas Weidenmaier, Christopher
author_sort	Winstel, Volker
title	Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization
title_short	Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization
title_full	Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization
title_fullStr	Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization
title_full_unstemmed	Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization
title_sort	wall teichoic acid glycosylation governs staphylococcus aureus nasal colonization
description	Nasal colonization by the human pathogen Staphylococcus aureus is a major risk factor for hospital- and community-acquired infections. A key factor required for nasal colonization is a cell surface-exposed zwitterionic glycopolymer, termed wall teichoic acid (WTA). However, the precise mechanisms that govern WTA-mediated nasal colonization have remained elusive. Here, we report that WTA GlcNAcylation is a pivotal requirement for WTA-dependent attachment of community-acquired methicillin-resistant S. aureus (MRSA) and emerging livestock-associated MRSA to human nasal epithelial cells, even under conditions simulating the nutrient composition and dynamic flow of nasal secretions. Depending on the S. aureus strain, WTA O-GlcNAcylation occurs in either α or β configuration, which have similar capacities to mediate attachment to human nasal epithelial cells, suggesting that many S. aureus strains maintain redundant pathways to ensure appropriate WTA glycosylation. Strikingly, a lack of WTA glycosylation significantly abrogated the ability of MRSA to colonize cotton rat nares in vivo. These results indicate that WTA glycosylation modulates S. aureus nasal colonization and may help to develop new strategies for eradicating S. aureus nasal colonization in the future.
publisher	American Society of Microbiology
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488942/
_version_	1613242931763740672

Wall Teichoic Acid Glycosylation Governs Staphylococcus aureus Nasal Colonization

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