Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation

Biogeochemical processes mediated by Fe(III)-reducing bacteria such as Shewanella oneidensis have the potential to influence the post-closure evolution of a geological disposal facility for radioactive wastes and to affect the solubility of some radionuclides. Furthermore, their potential to reduce...

Full description

Bibliographic Details
Main Authors:	Brown, Ashley R., Correa, Elon, Xu, Yun, AlMasoud, Najla, Pimblott, Simon M., Goodacre, Royston, Lloyd, Jonathan R.
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476702/

id	pubmed-4476702
recordtype	oai_dc
spelling	pubmed-44767022015-06-25 Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation Brown, Ashley R. Correa, Elon Xu, Yun AlMasoud, Najla Pimblott, Simon M. Goodacre, Royston Lloyd, Jonathan R. Research Article Biogeochemical processes mediated by Fe(III)-reducing bacteria such as Shewanella oneidensis have the potential to influence the post-closure evolution of a geological disposal facility for radioactive wastes and to affect the solubility of some radionuclides. Furthermore, their potential to reduce both Fe(III) and radionuclides can be harnessed for the bioremediation of radionuclide-contaminated land. As some such sites are likely to have significant radiation fluxes, there is a need to characterise the impact of radiation stress on such microorganisms. There have, however, been few global cell analyses on the impact of ionizing radiation on subsurface bacteria, so here we address the metabolic response of S. oneidensis MR-1 to acute doses of X-radiation. UV/Vis spectroscopy and CFU counts showed that although X-radiation decreased initial viability and extended the lag phase of batch cultures, final biomass yields remained unchanged. FT-IR spectroscopy of whole cells indicated an increase in lipid associated vibrations and decreases in vibrations tentatively assigned to nucleic acids, phosphate, saccharides and amines. MALDI-TOF-MS detected an increase in total protein expression in cultures exposed to 12 Gy. At 95 Gy, a decrease in total protein levels was generally observed, although an increase in a putative cold shock protein was observed, which may be related to the radiation stress response of this organism. Multivariate statistical analyses applied to these FT-IR and MALDI-TOF-MS spectral data suggested that an irradiated phenotype developed throughout subsequent generations. This study suggests that significant alteration to the metabolism of S. oneidensis MR-1 is incurred as a result of X-irradiation and that dose dependent changes to specific biomolecules characterise this response. Irradiated S. oneidensis also displayed enhanced levels of poorly crystalline Fe(III) oxide reduction, though the mechanism underpinning this phenomenon is unclear. Public Library of Science 2015-06-22 /pmc/articles/PMC4476702/ /pubmed/26098880 http://dx.doi.org/10.1371/journal.pone.0131249 Text en © 2015 Brown et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Brown, Ashley R. Correa, Elon Xu, Yun AlMasoud, Najla Pimblott, Simon M. Goodacre, Royston Lloyd, Jonathan R.
spellingShingle	Brown, Ashley R. Correa, Elon Xu, Yun AlMasoud, Najla Pimblott, Simon M. Goodacre, Royston Lloyd, Jonathan R. Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation
author_facet	Brown, Ashley R. Correa, Elon Xu, Yun AlMasoud, Najla Pimblott, Simon M. Goodacre, Royston Lloyd, Jonathan R.
author_sort	Brown, Ashley R.
title	Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation
title_short	Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation
title_full	Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation
title_fullStr	Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation
title_full_unstemmed	Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation
title_sort	phenotypic characterisation of shewanella oneidensis mr-1 exposed to x-radiation
description	Biogeochemical processes mediated by Fe(III)-reducing bacteria such as Shewanella oneidensis have the potential to influence the post-closure evolution of a geological disposal facility for radioactive wastes and to affect the solubility of some radionuclides. Furthermore, their potential to reduce both Fe(III) and radionuclides can be harnessed for the bioremediation of radionuclide-contaminated land. As some such sites are likely to have significant radiation fluxes, there is a need to characterise the impact of radiation stress on such microorganisms. There have, however, been few global cell analyses on the impact of ionizing radiation on subsurface bacteria, so here we address the metabolic response of S. oneidensis MR-1 to acute doses of X-radiation. UV/Vis spectroscopy and CFU counts showed that although X-radiation decreased initial viability and extended the lag phase of batch cultures, final biomass yields remained unchanged. FT-IR spectroscopy of whole cells indicated an increase in lipid associated vibrations and decreases in vibrations tentatively assigned to nucleic acids, phosphate, saccharides and amines. MALDI-TOF-MS detected an increase in total protein expression in cultures exposed to 12 Gy. At 95 Gy, a decrease in total protein levels was generally observed, although an increase in a putative cold shock protein was observed, which may be related to the radiation stress response of this organism. Multivariate statistical analyses applied to these FT-IR and MALDI-TOF-MS spectral data suggested that an irradiated phenotype developed throughout subsequent generations. This study suggests that significant alteration to the metabolism of S. oneidensis MR-1 is incurred as a result of X-irradiation and that dose dependent changes to specific biomolecules characterise this response. Irradiated S. oneidensis also displayed enhanced levels of poorly crystalline Fe(III) oxide reduction, though the mechanism underpinning this phenomenon is unclear.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476702/
_version_	1613238821023907840

Phenotypic Characterisation of Shewanella oneidensis MR-1 Exposed to X-Radiation

Similar Items