Quantitative Brightness Analysis of Fluorescence Intensity Fluctuations in E. Coli

The brightness measured by fluorescence fluctuation spectroscopy specifies the average stoichiometry of a labeled protein in a sample. Here we extended brightness analysis, which has been mainly applied in eukaryotic cells, to prokaryotic cells with E. coli serving as a model system. The small size...

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Bibliographic Details
Main Authors: Hur, Kwang-Ho, Mueller, Joachim D.
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476568/