A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals
Unique in vivo tests were conducted through the use of a fistulated ruminant, providing an ideal environment with a diverse and vibrant microbial community. Utilizing such a procedure can be especially invaluable for investigating the performance of antimicrobial materials related to human and anima...
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| Format: | Online |
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Nature Publishing Group
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476420/ |
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pubmed-4476420 |
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pubmed-44764202015-06-24 A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals Berean, Kyle J. Adetutu, Eric M. Zhen Ou, Jian Nour, Majid Nguyen, Emily P. Paull, David Mcleod, Jess Ramanathan, Rajesh Bansal, Vipul Latham, Kay Bishop-Hurley, Greg J. McSweeney, Chris Ball, Andrew S. Kalantar-zadeh, Kourosh Article Unique in vivo tests were conducted through the use of a fistulated ruminant, providing an ideal environment with a diverse and vibrant microbial community. Utilizing such a procedure can be especially invaluable for investigating the performance of antimicrobial materials related to human and animal related infections. In this pilot study, it is shown that the rumen of a fistulated animal provides an excellent live laboratory for assessing the properties of antimicrobial materials. We investigate microbial colonization onto model nanocomposites based on silver (Ag) nanoparticles at different concentrations into polydimethylsiloxane (PDMS). With implantable devices posing a major risk for hospital-acquired infections, the present study provides a viable solution to understand microbial colonization with the potential to reduce the incidence of infection through the introduction of Ag nanoparticles at the optimum concentrations. In vitro measurements were also conducted to show the validity of the approach. An optimal loading of 0.25 wt% Ag is found to show the greatest antimicrobial activity and observed through the in vivo tests to reduce the microbial diversity colonizing the surface. Nature Publishing Group 2015-06-22 /pmc/articles/PMC4476420/ /pubmed/26098413 http://dx.doi.org/10.1038/srep11515 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Berean, Kyle J. Adetutu, Eric M. Zhen Ou, Jian Nour, Majid Nguyen, Emily P. Paull, David Mcleod, Jess Ramanathan, Rajesh Bansal, Vipul Latham, Kay Bishop-Hurley, Greg J. McSweeney, Chris Ball, Andrew S. Kalantar-zadeh, Kourosh |
| spellingShingle |
Berean, Kyle J. Adetutu, Eric M. Zhen Ou, Jian Nour, Majid Nguyen, Emily P. Paull, David Mcleod, Jess Ramanathan, Rajesh Bansal, Vipul Latham, Kay Bishop-Hurley, Greg J. McSweeney, Chris Ball, Andrew S. Kalantar-zadeh, Kourosh A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals |
| author_facet |
Berean, Kyle J. Adetutu, Eric M. Zhen Ou, Jian Nour, Majid Nguyen, Emily P. Paull, David Mcleod, Jess Ramanathan, Rajesh Bansal, Vipul Latham, Kay Bishop-Hurley, Greg J. McSweeney, Chris Ball, Andrew S. Kalantar-zadeh, Kourosh |
| author_sort |
Berean, Kyle J. |
| title |
A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals |
| title_short |
A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals |
| title_full |
A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals |
| title_fullStr |
A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals |
| title_full_unstemmed |
A unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals |
| title_sort |
unique in vivo approach for investigating antimicrobial materials utilizing fistulated animals |
| description |
Unique in vivo tests were conducted through the use of a fistulated ruminant, providing an ideal environment with a diverse and vibrant microbial community. Utilizing such a procedure can be especially invaluable for investigating the performance of antimicrobial materials related to human and animal related infections. In this pilot study, it is shown that the rumen of a fistulated animal provides an excellent live laboratory for assessing the properties of antimicrobial materials. We investigate microbial colonization onto model nanocomposites based on silver (Ag) nanoparticles at different concentrations into polydimethylsiloxane (PDMS). With implantable devices posing a major risk for hospital-acquired infections, the present study provides a viable solution to understand microbial colonization with the potential to reduce the incidence of infection through the introduction of Ag nanoparticles at the optimum concentrations. In vitro measurements were also conducted to show the validity of the approach. An optimal loading of 0.25 wt% Ag is found to show the greatest antimicrobial activity and observed through the in vivo tests to reduce the microbial diversity colonizing the surface. |
| publisher |
Nature Publishing Group |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476420/ |
| _version_ |
1613238719557402624 |