Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae

Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae

F4 enterotoxigenic Escherichia coli (ETEC) cause diarrhoea and mortality in piglets leading to severe economic losses. Oral immunization of piglets with F4 fimbriae induces a protective intestinal immune response evidenced by an F4-specific serum and intestinal IgA response. However, successful oral...

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Main Authors: Nguyen, Ut V, Melkebeek, Vesna, Devriendt, Bert, Goetstouwers, Tiphanie, Van Poucke, Mario, Peelman, Luc, Goddeeris, Bruno M, Cox, Eric
Format: Online
Language:English
Published: BioMed Central 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476233/
id pubmed-4476233
recordtype oai_dc
spelling pubmed-44762332015-06-23 Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae Nguyen, Ut V Melkebeek, Vesna Devriendt, Bert Goetstouwers, Tiphanie Van Poucke, Mario Peelman, Luc Goddeeris, Bruno M Cox, Eric Research Article F4 enterotoxigenic Escherichia coli (ETEC) cause diarrhoea and mortality in piglets leading to severe economic losses. Oral immunization of piglets with F4 fimbriae induces a protective intestinal immune response evidenced by an F4-specific serum and intestinal IgA response. However, successful oral immunization of pigs with F4 fimbriae in the presence of maternal immunity has not been demonstrated yet. In the present study we aimed to evaluate the effect of maternal immunity on the induction of a systemic immune response upon oral immunization of piglets. Whereas F4-specific IgG and IgA could be induced by oral immunization of pigs without maternal antibodies and by intramuscular immunization of pigs with maternal antibodies, no such response was seen in the orally immunized animals with maternal antibodies. Since maternal antibodies can mask an antibody response, we also looked by ELIspot assays for circulating F4-specific antibody secreting cells (ASCs). Enumerating the F4-specific ASCs within the circulating peripheral blood mononuclear cells, and the number of F4-specific IgA ASCs within the circulating IgA+ B-cells revealed an F4-specific immune response in the orally immunized animals with maternal antibodies. Interestingly, results suggest a more robust IgA booster response by oral immunization of pigs with than without maternal antibodies. These results demonstrate that oral immunization of piglets with F4-specific maternal antibodies is feasible and that these maternal antibodies seem to enhance the secondary systemic immune response. Furthermore, our ELIspot assay on enriched IgA+ B-cells could be used as a screening procedure to optimize mucosal immunization protocols in pigs with maternal immunity. BioMed Central 2015-06-23 2015 /pmc/articles/PMC4476233/ /pubmed/26100608 http://dx.doi.org/10.1186/s13567-015-0210-3 Text en © Nguyen et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Nguyen, Ut V
Melkebeek, Vesna
Devriendt, Bert
Goetstouwers, Tiphanie
Van Poucke, Mario
Peelman, Luc
Goddeeris, Bruno M
Cox, Eric
spellingShingle Nguyen, Ut V
Melkebeek, Vesna
Devriendt, Bert
Goetstouwers, Tiphanie
Van Poucke, Mario
Peelman, Luc
Goddeeris, Bruno M
Cox, Eric
Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae
author_facet Nguyen, Ut V
Melkebeek, Vesna
Devriendt, Bert
Goetstouwers, Tiphanie
Van Poucke, Mario
Peelman, Luc
Goddeeris, Bruno M
Cox, Eric
author_sort Nguyen, Ut V
title Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae
title_short Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae
title_full Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae
title_fullStr Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae
title_full_unstemmed Maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with F4 fimbriae
title_sort maternal immunity enhances systemic recall immune responses upon oral immunization of piglets with f4 fimbriae
description F4 enterotoxigenic Escherichia coli (ETEC) cause diarrhoea and mortality in piglets leading to severe economic losses. Oral immunization of piglets with F4 fimbriae induces a protective intestinal immune response evidenced by an F4-specific serum and intestinal IgA response. However, successful oral immunization of pigs with F4 fimbriae in the presence of maternal immunity has not been demonstrated yet. In the present study we aimed to evaluate the effect of maternal immunity on the induction of a systemic immune response upon oral immunization of piglets. Whereas F4-specific IgG and IgA could be induced by oral immunization of pigs without maternal antibodies and by intramuscular immunization of pigs with maternal antibodies, no such response was seen in the orally immunized animals with maternal antibodies. Since maternal antibodies can mask an antibody response, we also looked by ELIspot assays for circulating F4-specific antibody secreting cells (ASCs). Enumerating the F4-specific ASCs within the circulating peripheral blood mononuclear cells, and the number of F4-specific IgA ASCs within the circulating IgA+ B-cells revealed an F4-specific immune response in the orally immunized animals with maternal antibodies. Interestingly, results suggest a more robust IgA booster response by oral immunization of pigs with than without maternal antibodies. These results demonstrate that oral immunization of piglets with F4-specific maternal antibodies is feasible and that these maternal antibodies seem to enhance the secondary systemic immune response. Furthermore, our ELIspot assay on enriched IgA+ B-cells could be used as a screening procedure to optimize mucosal immunization protocols in pigs with maternal immunity.
publisher BioMed Central
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4476233/
_version_ 1613238671518990336

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