A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs

Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo....

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Main Authors:	Fulga, Tudor A., McNeill, Elizabeth M., Binari, Richard, Yelick, Julia, Blanche, Alexandra, Booker, Matthew, Steinkraus, Bruno R., Schnall-Levin, Michael, Zhao, Yong, DeLuca, Todd, Bejarano, Fernando, Han, Zhe, Lai, Eric C., Wall, Dennis P., Perrimon, Norbert, Van Vactor, David
Format:	Online
Language:	English
Published:	Nature Pub. Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471878/

id	pubmed-4471878
recordtype	oai_dc
spelling	pubmed-44718782015-09-11 A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs Fulga, Tudor A. McNeill, Elizabeth M. Binari, Richard Yelick, Julia Blanche, Alexandra Booker, Matthew Steinkraus, Bruno R. Schnall-Levin, Michael Zhao, Yong DeLuca, Todd Bejarano, Fernando Han, Zhe Lai, Eric C. Wall, Dennis P. Perrimon, Norbert Van Vactor, David Article Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo. However, the application of these loss-of-function strategies has been limited. Here we offer a comprehensive library of 141 conditional miRNA sponges targeting well-conserved miRNAs in Drosophila. Ubiquitous miRNA sponge delivery and consequent systemic miRNA inhibition uncovers a relatively small number of miRNA families underlying viability and gross morphogenesis, with false discovery rates in the 4–8% range. In contrast, tissue-specific silencing of muscle-enriched miRNAs reveals a surprisingly large number of novel miRNA contributions to the maintenance of adult indirect flight muscle structure and function. A strong correlation between miRNA abundance and physiological relevance is not observed, underscoring the importance of unbiased screens when assessing the contributions of miRNAs to complex biological processes. Nature Pub. Group 2015-06-17 /pmc/articles/PMC4471878/ /pubmed/26081261 http://dx.doi.org/10.1038/ncomms8279 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Fulga, Tudor A. McNeill, Elizabeth M. Binari, Richard Yelick, Julia Blanche, Alexandra Booker, Matthew Steinkraus, Bruno R. Schnall-Levin, Michael Zhao, Yong DeLuca, Todd Bejarano, Fernando Han, Zhe Lai, Eric C. Wall, Dennis P. Perrimon, Norbert Van Vactor, David
spellingShingle	Fulga, Tudor A. McNeill, Elizabeth M. Binari, Richard Yelick, Julia Blanche, Alexandra Booker, Matthew Steinkraus, Bruno R. Schnall-Levin, Michael Zhao, Yong DeLuca, Todd Bejarano, Fernando Han, Zhe Lai, Eric C. Wall, Dennis P. Perrimon, Norbert Van Vactor, David A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs
author_facet	Fulga, Tudor A. McNeill, Elizabeth M. Binari, Richard Yelick, Julia Blanche, Alexandra Booker, Matthew Steinkraus, Bruno R. Schnall-Levin, Michael Zhao, Yong DeLuca, Todd Bejarano, Fernando Han, Zhe Lai, Eric C. Wall, Dennis P. Perrimon, Norbert Van Vactor, David
author_sort	Fulga, Tudor A.
title	A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs
title_short	A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs
title_full	A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs
title_fullStr	A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs
title_full_unstemmed	A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs
title_sort	transgenic resource for conditional competitive inhibition of conserved drosophila micrornas
description	Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo. However, the application of these loss-of-function strategies has been limited. Here we offer a comprehensive library of 141 conditional miRNA sponges targeting well-conserved miRNAs in Drosophila. Ubiquitous miRNA sponge delivery and consequent systemic miRNA inhibition uncovers a relatively small number of miRNA families underlying viability and gross morphogenesis, with false discovery rates in the 4–8% range. In contrast, tissue-specific silencing of muscle-enriched miRNAs reveals a surprisingly large number of novel miRNA contributions to the maintenance of adult indirect flight muscle structure and function. A strong correlation between miRNA abundance and physiological relevance is not observed, underscoring the importance of unbiased screens when assessing the contributions of miRNAs to complex biological processes.
publisher	Nature Pub. Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471878/
_version_	1613237205197651968

A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs

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