Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides

Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum, the progenitor of durum and bread wheat, and maintains a rich allelic diversity among its wild populations. The lack of adequate genetic and genomic resources, however, restricts its exploitation in wheat...

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Main Authors:	Ani Akpinar, Bala, Yuce, Meral, Lucas, Stuart, Vrána, Jan, Burešová, Veronika, Doležel, Jaroslav, Budak, Hikmet
Format:	Online
Language:	English
Published:	Nature Publishing Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471722/

id	pubmed-4471722
recordtype	oai_dc
spelling	pubmed-44717222015-06-30 Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides Ani Akpinar, Bala Yuce, Meral Lucas, Stuart Vrána, Jan Burešová, Veronika Doležel, Jaroslav Budak, Hikmet Article Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum, the progenitor of durum and bread wheat, and maintains a rich allelic diversity among its wild populations. The lack of adequate genetic and genomic resources, however, restricts its exploitation in wheat improvement. Here, we report next-generation sequencing of the flow-sorted chromosome 5B of T. dicoccoides to shed light into its genome structure, function and organization by exploring the repetitive elements, protein-encoding genes and putative microRNA and tRNA coding sequences. Comparative analyses with its counterparts in modern and wild wheats suggest clues into the B-genome evolution. Syntenic relationships of chromosome 5B with the model grasses can facilitate further efforts for fine-mapping of traits of interest. Mapping of 5B sequences onto the root transcriptomes of two additional T. dicoccoides genotypes, with contrasting drought tolerances, revealed several thousands of single nucleotide polymorphisms, of which 584 shared polymorphisms on 228 transcripts were specific to the drought-tolerant genotype. To our knowledge, this study presents the largest genomics resource currently available for T. dicoccoides, which, we believe, will encourage the exploitation of its genetic and genomic potential for wheat improvement to meet the increasing demand to feed the world. Nature Publishing Group 2015-06-18 /pmc/articles/PMC4471722/ /pubmed/26084265 http://dx.doi.org/10.1038/srep10763 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ani Akpinar, Bala Yuce, Meral Lucas, Stuart Vrána, Jan Burešová, Veronika Doležel, Jaroslav Budak, Hikmet
spellingShingle	Ani Akpinar, Bala Yuce, Meral Lucas, Stuart Vrána, Jan Burešová, Veronika Doležel, Jaroslav Budak, Hikmet Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides
author_facet	Ani Akpinar, Bala Yuce, Meral Lucas, Stuart Vrána, Jan Burešová, Veronika Doležel, Jaroslav Budak, Hikmet
author_sort	Ani Akpinar, Bala
title	Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides
title_short	Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides
title_full	Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides
title_fullStr	Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides
title_full_unstemmed	Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides
title_sort	molecular organization and comparative analysis of chromosome 5b of the wild wheat ancestor triticum dicoccoides
description	Wild emmer wheat, Triticum turgidum ssp. dicoccoides is the wild relative of Triticum turgidum, the progenitor of durum and bread wheat, and maintains a rich allelic diversity among its wild populations. The lack of adequate genetic and genomic resources, however, restricts its exploitation in wheat improvement. Here, we report next-generation sequencing of the flow-sorted chromosome 5B of T. dicoccoides to shed light into its genome structure, function and organization by exploring the repetitive elements, protein-encoding genes and putative microRNA and tRNA coding sequences. Comparative analyses with its counterparts in modern and wild wheats suggest clues into the B-genome evolution. Syntenic relationships of chromosome 5B with the model grasses can facilitate further efforts for fine-mapping of traits of interest. Mapping of 5B sequences onto the root transcriptomes of two additional T. dicoccoides genotypes, with contrasting drought tolerances, revealed several thousands of single nucleotide polymorphisms, of which 584 shared polymorphisms on 228 transcripts were specific to the drought-tolerant genotype. To our knowledge, this study presents the largest genomics resource currently available for T. dicoccoides, which, we believe, will encourage the exploitation of its genetic and genomic potential for wheat improvement to meet the increasing demand to feed the world.
publisher	Nature Publishing Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471722/
_version_	1613237156404264960

Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides

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