Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family
Aquaporins (AQPs) are major intrinsic proteins (MIPs) that mediate bidirectional flux of water and other substrates across cell membranes, and play critical roles in plant-water relations, dehydration stress responses and crop productivity. However, limited data are available as yet on the contribut...
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Public Library of Science
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461243/ |
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pubmed-44612432015-06-16 Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family Hove, Runyararo M. Ziemann, Mark Bhave, Mrinal Research Article Aquaporins (AQPs) are major intrinsic proteins (MIPs) that mediate bidirectional flux of water and other substrates across cell membranes, and play critical roles in plant-water relations, dehydration stress responses and crop productivity. However, limited data are available as yet on the contributions of these proteins to the physiology of the major crop barley (Hordeum vulgare). The present work reports the identification and expression analysis of the barley MIP family. A comprehensive search of publicly available leaf mRNA-seq data, draft barley genome data, GenBank transcripts and sixteen new annotations together revealed that the barley MIP family is comprised of at least forty AQPs. Alternative splicing events were likely in two plasma membrane intrinsic protein (PIP) AQPs. Analyses of the AQP signature sequences and specificity determining positions indicated a potential of several putative AQP isoforms to transport non-aqua substrates including physiological important substrates, and respond to abiotic stresses. Analysis of our publicly available leaf mRNA-seq data identified notable differential expression of HvPIP1;2 and HvTIP4;1 under salt stress. Analyses of other gene expression resources also confirmed isoform-specific responses in different tissues and/or in response to salinity, as well as some potentially inter-cultivar differences. The work reports systematic and comprehensive analysis of most, if not all, barley AQP genes, their sequences, expression patterns in different tissues, potential transport and stress response functions, and a strong framework for selection and/or development of stress tolerant barley varieties. In addition, the barley data would be highly valuable for genetic studies of the evolutionarily closely related wheat (Triticum aestivum L.). Public Library of Science 2015-06-09 /pmc/articles/PMC4461243/ /pubmed/26057533 http://dx.doi.org/10.1371/journal.pone.0128025 Text en © 2015 Hove et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Hove, Runyararo M. Ziemann, Mark Bhave, Mrinal |
| spellingShingle |
Hove, Runyararo M. Ziemann, Mark Bhave, Mrinal Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family |
| author_facet |
Hove, Runyararo M. Ziemann, Mark Bhave, Mrinal |
| author_sort |
Hove, Runyararo M. |
| title |
Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family |
| title_short |
Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family |
| title_full |
Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family |
| title_fullStr |
Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family |
| title_full_unstemmed |
Identification and Expression Analysis of the Barley (Hordeum vulgare L.) Aquaporin Gene Family |
| title_sort |
identification and expression analysis of the barley (hordeum vulgare l.) aquaporin gene family |
| description |
Aquaporins (AQPs) are major intrinsic proteins (MIPs) that mediate bidirectional flux of water and other substrates across cell membranes, and play critical roles in plant-water relations, dehydration stress responses and crop productivity. However, limited data are available as yet on the contributions of these proteins to the physiology of the major crop barley (Hordeum vulgare). The present work reports the identification and expression analysis of the barley MIP family. A comprehensive search of publicly available leaf mRNA-seq data, draft barley genome data, GenBank transcripts and sixteen new annotations together revealed that the barley MIP family is comprised of at least forty AQPs. Alternative splicing events were likely in two plasma membrane intrinsic protein (PIP) AQPs. Analyses of the AQP signature sequences and specificity determining positions indicated a potential of several putative AQP isoforms to transport non-aqua substrates including physiological important substrates, and respond to abiotic stresses. Analysis of our publicly available leaf mRNA-seq data identified notable differential expression of HvPIP1;2 and HvTIP4;1 under salt stress. Analyses of other gene expression resources also confirmed isoform-specific responses in different tissues and/or in response to salinity, as well as some potentially inter-cultivar differences. The work reports systematic and comprehensive analysis of most, if not all, barley AQP genes, their sequences, expression patterns in different tissues, potential transport and stress response functions, and a strong framework for selection and/or development of stress tolerant barley varieties. In addition, the barley data would be highly valuable for genetic studies of the evolutionarily closely related wheat (Triticum aestivum L.). |
| publisher |
Public Library of Science |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461243/ |
| _version_ |
1613233622591995904 |