The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?

The presence of distinct electrophysiological pathways within the atrioventricular node (AVN) is a prerequisite for atrioventricular nodal reentrant tachycardia to occur. In this study, the different cell contributions that may account for the anatomical and functional heterogeneity of the AVN were...

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Main Authors:	Kelder, Tim P, Vicente-Steijn, Rebecca, Harryvan, Tom J, Kosmidis, Georgios, Gittenberger-de Groot, Adriana C, Poelmann, Rob E, Schalij, Martin J, DeRuiter, Marco C, Jongbloed, Monique RM
Format:	Online
Language:	English
Published:	BlackWell Publishing Ltd 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459851/

id	pubmed-4459851
recordtype	oai_dc
spelling	pubmed-44598512015-06-16 The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development? Kelder, Tim P Vicente-Steijn, Rebecca Harryvan, Tom J Kosmidis, Georgios Gittenberger-de Groot, Adriana C Poelmann, Rob E Schalij, Martin J DeRuiter, Marco C Jongbloed, Monique RM Original Articles The presence of distinct electrophysiological pathways within the atrioventricular node (AVN) is a prerequisite for atrioventricular nodal reentrant tachycardia to occur. In this study, the different cell contributions that may account for the anatomical and functional heterogeneity of the AVN were investigated. To study the temporal development of the AVN, the expression pattern of ISL1, expressed in cardiac progenitor cells, was studied in sequential stages performing co-staining with myocardial markers (TNNI2 and NKX2-5) and HCN4 (cardiac conduction system marker). An ISL1+/TNNI2+/HCN4+ continuity between the myocardium of the sinus venosus and atrioventricular canal was identified in the region of the putative AVN, which showed a pacemaker-like phenotype based on single cell patch-clamp experiments. Furthermore, qPCR analysis showed that even during early development, different cell populations can be identified in the region of the putative AVN. Fate mapping was performed by in ovo vital dye microinjection. Embryos were harvested and analysed 24 and 48 hrs post-injection. These experiments showed incorporation of sinus venosus myocardium in the posterior region of the atrioventricular canal. The myocardium of the sinus venosus contributes to the atrioventricular canal. It is postulated that the myocardium of the sinus venosus contributes to nodal extensions or transitional cells of the AVN since these cells are located in the posterior region of the AVN. This finding may help to understand the origin of atrioventricular nodal reentrant tachycardia. BlackWell Publishing Ltd 2015-06 2015-03-06 /pmc/articles/PMC4459851/ /pubmed/25752780 http://dx.doi.org/10.1111/jcmm.12525 Text en © 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Kelder, Tim P Vicente-Steijn, Rebecca Harryvan, Tom J Kosmidis, Georgios Gittenberger-de Groot, Adriana C Poelmann, Rob E Schalij, Martin J DeRuiter, Marco C Jongbloed, Monique RM
spellingShingle	Kelder, Tim P Vicente-Steijn, Rebecca Harryvan, Tom J Kosmidis, Georgios Gittenberger-de Groot, Adriana C Poelmann, Rob E Schalij, Martin J DeRuiter, Marco C Jongbloed, Monique RM The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
author_facet	Kelder, Tim P Vicente-Steijn, Rebecca Harryvan, Tom J Kosmidis, Georgios Gittenberger-de Groot, Adriana C Poelmann, Rob E Schalij, Martin J DeRuiter, Marco C Jongbloed, Monique RM
author_sort	Kelder, Tim P
title	The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
title_short	The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
title_full	The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
title_fullStr	The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
title_full_unstemmed	The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
title_sort	sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?
description	The presence of distinct electrophysiological pathways within the atrioventricular node (AVN) is a prerequisite for atrioventricular nodal reentrant tachycardia to occur. In this study, the different cell contributions that may account for the anatomical and functional heterogeneity of the AVN were investigated. To study the temporal development of the AVN, the expression pattern of ISL1, expressed in cardiac progenitor cells, was studied in sequential stages performing co-staining with myocardial markers (TNNI2 and NKX2-5) and HCN4 (cardiac conduction system marker). An ISL1+/TNNI2+/HCN4+ continuity between the myocardium of the sinus venosus and atrioventricular canal was identified in the region of the putative AVN, which showed a pacemaker-like phenotype based on single cell patch-clamp experiments. Furthermore, qPCR analysis showed that even during early development, different cell populations can be identified in the region of the putative AVN. Fate mapping was performed by in ovo vital dye microinjection. Embryos were harvested and analysed 24 and 48 hrs post-injection. These experiments showed incorporation of sinus venosus myocardium in the posterior region of the atrioventricular canal. The myocardium of the sinus venosus contributes to the atrioventricular canal. It is postulated that the myocardium of the sinus venosus contributes to nodal extensions or transitional cells of the AVN since these cells are located in the posterior region of the AVN. This finding may help to understand the origin of atrioventricular nodal reentrant tachycardia.
publisher	BlackWell Publishing Ltd
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459851/
_version_	1613233092742348800

The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?

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